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Co-consumption of glucose and xylose for organic acid production by Aspergillus carbonarius cultivated in wheat straw hydrolysate

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Abstract

Aspergillus carbonarius exhibits excellent abilities to utilize a wide range of carbon sources and to produce various organic acids. In this study, wheat straw hydrolysate containing high concentrations of glucose and xylose was used for organic acid production by A. carbonarius. The results indicated that A. carbonarius efficiently co-consumed glucose and xylose and produced various types of organic acids in hydrolysate adjusted to pH 7. The inhibitor tolerance of A. carbonarius to the hydrolysate at different pH values was investigated and compared using spores and recycled mycelia. This comparison showed a slight difference in the inhibitor tolerance of the spores and the recycled mycelia based on their growth patterns. Moreover, the wild-type and a glucose oxidase deficient (Δgox) mutant were compared for their abilities to produce organic acids using the hydrolysate and a defined medium. The two strains showed a different pattern of organic acid production in the hydrolysate where the Δgox mutant produced more oxalic acid but less citric acid than the wild-type, which was different from the results obtained in the defined medium This study demonstrates the feasibility of using lignocellulosic biomass for the organic acid production by A. carbonarius.

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Acknowledgments

Financial support from the Danish Strategic Research Program MycoFuelChem (DSF Grant No. 11-116803) is acknowledged. We thank laboratory technician Gitte Hinz-Berg for HPLC analysis.

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  1. Konstantinos Souroullas

    Present address: MEDOCHEMIE LTD, 1-10 Constantinoupoleos St., 3011, Limassol, Cyprus

Authors and Affiliations

  1. Section for Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University Copenhagen, A. C. Meyers Vaenge 15, 2450, Copenhagen SV, Denmark

    Lei Yang, Mette Lübeck, Konstantinos Souroullas & Peter S. Lübeck

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  1. Lei Yang
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  2. Mette Lübeck
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  3. Konstantinos Souroullas
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Correspondence to Peter S. Lübeck.

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This study does not contain any experiment with human participants or animals performed by any of the authors.

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Yang, L., Lübeck, M., Souroullas, K. et al. Co-consumption of glucose and xylose for organic acid production by Aspergillus carbonarius cultivated in wheat straw hydrolysate. World J Microbiol Biotechnol 32, 57 (2016). https://doi.org/10.1007/s11274-016-2025-4

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