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Reclamation of petrol oil contaminated soil by rhamnolipids producing PGPR strains for growing Withania somnifera a medicinal shrub

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Abstract

Soil contaminated by hydrocarbons, cannot be used for agricultural intents due to their toxic effect to the plants. Surfactants producing by plant growth promotory rhizobacteria (PGPR) can effectively rig the problem of petroleum hydrocarbon contamination and growth promotion on such contaminated soils. In the present study three Pseudomonas strains isolated from contaminated soil identified by 16S rRNA analysis were ascertained for PGPR as well as biosurfactants property. Biosurfactants produced by the strains were further characterized and essayed for rhamnolipids. Inoculation of the strains in petrol hydrocarbon contaminated soil and its interaction with Withania somnifera in presence of petrol oil hydrocarbons depict that the strains helped in growth promotion of Withania somnifera in petrol oil contaminated soil while rhamnolipids helped in lowering the toxicity of petrol oil. The study was found to be beneficial as the growth and antioxidant activity of Withania sominfera was enhanced. Hence the present study signifies that rhamnolipids producing PGPR strains could be a better measure for reclamation of petrol contaminated sites for growing medicinal plants.

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Authors and Affiliations

  1. Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar (A Central) University, Vidya Vihar, Raibareli Road, Lucknow, 226025, India

    Rajesh Kumar & Amar Jyoti Das

  2. Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, India

    Rajesh Kumar

  3. Eco-Restoration Division, National Environmental Engineering Research Institute, Nagpur, 440020, India

    Asha A. Juwarkar

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  1. Rajesh Kumar
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  2. Amar Jyoti Das
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  3. Asha A. Juwarkar
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Correspondence to Rajesh Kumar.

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Kumar, R., Das, A.J. & Juwarkar, A.A. Reclamation of petrol oil contaminated soil by rhamnolipids producing PGPR strains for growing Withania somnifera a medicinal shrub. World J Microbiol Biotechnol 31, 307–313 (2015). https://doi.org/10.1007/s11274-014-1782-1

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  • DOI: https://doi.org/10.1007/s11274-014-1782-1

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