Abstract
High-performance liquid chromatography was used to separate Cr(III) and Cr(VI) in samples with detection by inductively coupled plasma mass spectrometry(ICP-MS). The separation was achieved on a weak anion exchange column. The mobile phase was pH 7.0 ammonium nitrate solution. The redox reaction between Cr(III) and Cr(VI) was avoided during separation and determination. This separation method could be used to separate the samples with large concentration differences between Cr(III) and Cr(VI). The alkaline digestion was used to extract chromium in solid sample, which had no effect on the retention time and the peak area of the Cr(VI). However, the conversion of Cr(VI) from Cr(III) was observed during alkaline digestion, which displayed positive relation with the ratio of Cr(III) and Cr(VI) in samples. Both Cr(III) and Cr(VI) contents of chromium yeasts cultured in media with different chromium additions were determined. The spike recoveries of Cr(VI) for chromium yeasts were in the range of 95–108 %.
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Acknowledgments
This work was supported by the CAS Agenda to Provide S&T Support and Services for National Strategic Emerging Industries (project no.Y3HX121HX1). The authors wish to thank Juane Song (Agilent Technologies Beijing, China) for helpful suggestions during the course of this work.
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The authors declare there no conflict of interest regarding this manuscript. This article does not contain any studies with human or animal subjects.
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Guo, X., Liu, W., Bai, X. et al. Speciation of chromium in chromium yeast. World J Microbiol Biotechnol 30, 3245–3250 (2014). https://doi.org/10.1007/s11274-014-1751-8
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DOI: https://doi.org/10.1007/s11274-014-1751-8