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Recombinant xylanase from Streptomyces coelicolor Ac-738: characterization and the effect on xylan-containing products

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Abstract

A xylanase gene was isolated from the genomic DNA of Streptomyces coelicolor Ac-738. The 723-bp full-length gene encoded a 241-amino acid peptide consisting of a 49-residue putative TAT signal peptide and a glycoside hydrolase family-11 domain. The mature enzyme called XSC738 was expressed in Escherichia coli M15[pREP4]. The electrophoretically homogeneous protein with a specific activity of 167 U/mg for beechwood xylan was purified. The pH optimum of XSC738 was at pH 6; a high activity was retained within a pH range of 4.5–8.5. The enzyme was thermostable at 50–60 °C and retained an activity at pH 3.0–7.0. Xylanase XSC738 was activated by Mn2+, Co2+ and largely inhibited by Cd2+, SDS and EDTA. The products of xylan hydrolysis were mainly xylobiose, xylotriose, xylopentaose and xylohexose. Xylotetraose appeared as a minor product. Processing of such agricultural xylan-containing products as wheat, oats, soy flour and wheat bran by xylanase resulted in an increased content of sugars.

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References

  • Bajpai P (1999) Application of enzymes in the pulp and paper industry. Biotechnol Prog 15:147–157

    Article  CAS  Google Scholar 

  • Biely P, Vrsanskh M, Tenkanen M, Kluepfel D (1997) Endo-β-1,4-xylanase families: differences in catalytic properties. J Biotechnol 57:151–166

    Article  CAS  Google Scholar 

  • Britton HTS, Robinson RA (1931) Universal buffer solutions and the dissociation constant of veronal. J Chem Soc 1456–1462. http://pubs.rsc.org/en/Content/ArticleLanding/1931/JR/jr9310001456#!divAbstract

  • Butt MS, Tahir-Nadeem M, Ahmad Z, Sultan MT (2008) Xylanases and their applications in baking industry. Food Technol Biotechnol 46:22–31

    CAS  Google Scholar 

  • Choct M, Dersjant-Li Y, McLeish J, Peisker M (2010) Soy oligosaccharides and soluble non-starch polysaccharides: a review of digestion, nutritive and anti-nutritive effects in pigs and poultry. Asian-Australas J Anim Sci 23:1386–1398

    Article  CAS  Google Scholar 

  • Clarke JH, Rixon JE, Ciruela A, Gilbert HJ, Hazlewood GP (1997) Family-10 and Family-11 xylanases differ in their capacity to enhance the bleachability of hardwood and softwood paper pulps. Appl Microbiol Biotechnol 48:177–183

    Article  CAS  Google Scholar 

  • Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29:3–23

    Article  CAS  Google Scholar 

  • Damaso MC, de Castro AM, Castro RM, Andrade CM, Pereira N Jr (2004) Application of xylanase from Thermomyces lanuginosus IOC-4145 for enzymatic hydrolysis of corncob and sugarcane bagasse. Appl Biochem Biotechnol 113–116:1003–1012

    Article  Google Scholar 

  • Deesukon W, Nishimura Y, Sakamoto T, Sukhumsirichart W (2012) Purification, characterization of GH11 endo-β-1,4-xylanase from thermotolerant Streptomyces sp. SWU10 and overexpression in Pichia pastoris KM71H. Mol Biotechnol. doi:10.1007/s12033-012-9541-8

    Google Scholar 

  • Deesukona W, Nishimurab Y, Haradab N, Sakamotob T, Sukhumsirichart W (2011) Purification, characterization and gene cloning of two forms of a thermostable endo-xylanase from Streptomyces sp. SWU10. Process Biochem 46:2255–2262

    Article  Google Scholar 

  • Ebringerova A (2006) Structural diversity and application potential of hemicelluloses. Macromol Symp 232:1–12

    Article  CAS  Google Scholar 

  • Englyst H (1989) Classification and measurement of plant polysaccharides. Anim Feed Sci Technol 23:27–42

    Article  CAS  Google Scholar 

  • Gebruers K, Dornez E, Bedo Z, Rakszegi M, Courtin CM, Delcour JA (2010) Variability in xylanase and xylanase inhibition activities in different cereals in the HEALTHGRAIN diversity screen and contribution of environment and genotype to this variability in common wheat. J Agric Food Chem 58:9362–9371

    Article  CAS  Google Scholar 

  • Georis J, Giannotta F, De Buyl E, Granier B, Frere J-M (2000) Purification and properties of three endo-b-1,4-xylanases produced by Streptomyces sp. strain S38 which differ in their ability to enhance the bleaching of kraft pulps. Enzyme Microb Technol 26:178–186

    Article  CAS  Google Scholar 

  • Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268

    Article  CAS  Google Scholar 

  • Henrissat BA (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 280:309–316

    CAS  Google Scholar 

  • Kaneko S, Kuno A, Muramatsu M, Iwamatsu S, Kusakabe I, Hayashi K (2000) Purification and characterization of a family G/11 beta-xylanase from Streptomyces olivaceoviridis E-86. Biosci Biotechnol Biochem 64:447–451

    Article  CAS  Google Scholar 

  • Kim JC, Simmins PH, Mullan BP, Pluske JR (2005) The digestible energy value of wheat for pigs, with special reference to the post-weaned animal (Review). Anim Feed Sci Technol 122:257–287

    Article  Google Scholar 

  • Kim DY, Han MK, Oh H-W, Park D-S, Kim S-J, Lee S-G, Shin D-H, Son K-H, Bae KS, Park H-Y (2010) Catalytic properties of a GH10 endo-β-1,4-xylanase from Streptomyces thermocarboxydus HY-15 isolated from the gut of Eisenia fetida. J Mol Catal B Enzym 62:32–39

    Google Scholar 

  • Kluepfel D, Vats-Mehta S, Aumont F, Shareck F, Morosoli R (1990) Purification and characterization of a new xylanase (xylanase B) produced by Streptomyces lividans 66. Biochem J 267:45–50

    CAS  Google Scholar 

  • Kluepfel D, Daigneault N, Morosoli R, Shareck F (1992) Purification and characterization of a new xylanase (xylanase C) produced by Streptomyces lividans 66. Appl Microbiol Biotechnol 36:626–631

    Article  CAS  Google Scholar 

  • Laemmly UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  Google Scholar 

  • Li X, She Y, Sun B, Song H, Zhu Y, Lv Y, Song H (2010) Purification and characterization of a cellulase-free, thermostable xylanase from Streptomyces rameus L2001 and its biobleaching effect on wheat straw pulp. Biochem Engin J 52:71–78

    Article  CAS  Google Scholar 

  • Li X, Li E, Zhu Y, Teng C, Sun B, Song H, Yang R (2012) A typical endo-xylanase from Streptomyces rameus L2001 and its unique characteristics in xylooligosaccharide production. Carbohydr Res 359:30–36

    Article  CAS  Google Scholar 

  • Ninawe S, Kapoor M, Kuhad RC (2008) Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32. Bioresour Technol 99:1252–1258

    Article  CAS  Google Scholar 

  • Pirgozliev V, Bedford MR, Acamovic T (2010) Effect of dietary xylanase on energy, amino acid and mineral metabolism, and egg production and quality in laying hens. Br Poult Sci 51:639–647

    Article  CAS  Google Scholar 

  • Qiu Z, Shi P, Luo H, Bai Y, Yuan T, Yang P, Liu S, Yao B (2010) A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry. Enzyme Microb Technol 46:506–512

    Article  CAS  Google Scholar 

  • Saha BC (2003) Purification and properties of an extracellular b-xylosidase from a newly isolated Fusarium proliferatum. Bioresour Technol 90:33–38

    Article  CAS  Google Scholar 

  • Saha BC, Bothast RJ (1999) Enzymology of xylan degradation. Biopolymers. ACS Symposium Series 723:167–194

  • Scheller HV, Ulvskov P (2010) Hemicelluloses. Annu Rev Plant Biol 61:263–289

    Article  CAS  Google Scholar 

  • Shareck F, Roy C, Yaguchi M, Morosoli R, Kluepfel D (1991) Sequences of three genes specifying xylanases in Streptomyces lividans. Gene 107:75–82

    Article  CAS  Google Scholar 

  • Shi P, Qiu Z, Bai Y, Yuan T, Huang H, Pan X, Yang P, Zhang W, Yao B (2012) A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose. World J Microbiol Biotechnol 28:687–692

    Google Scholar 

  • Silversides FG, Scott TA, Korver DR, Afsharmanesh M, Hruby M (2006) A study on the interaction of xylanase and phytase enzymes in wheat-based diets fed to commercial white and brown egg laying hens. Poult Sci 85:297–305

    Article  CAS  Google Scholar 

  • Simkhada JR, Yoo HY, Choi YH, Kim SW, Yoo JC (2012) An extremely alkaline novel xylanase from a newly isolated Streptomyces strain cultivated in corncob medium. Appl Biochem Biotechnol 168:2017–2027

    Article  CAS  Google Scholar 

  • Yan Q, Hao S, Jiang Z, Zhai Q, Chen W (2009) Properties of a xylanase from Streptomyces matensis being suitable for xylooligosaccharides production. J Mol Catal B Enzym 58:72–77

    Article  CAS  Google Scholar 

  • Yanga H, Wanga K, Songa X, Xu F (2011) Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa. Bioresour Technol 102:7171–7176

    Article  Google Scholar 

  • Zhou J, Shi P, Zhang R, Huang H, Meng K, Yang P, Yao B (2011) Symbiotic Streptomyces sp. TN119 GH 11 xylanase: a new pH-stable, protease- and SDS-resistant xylanase. J Ind Microbiol Biotechnol 38:523–530

    Article  CAS  Google Scholar 

  • Zhu Y, Li X, Sun B, Song H, Li E, Song H (2012) Properties of an alkaline-tolerant, thermostable xylanase from Streptomyces chartreusis L1105, suitable for xylooligosaccharide production. J Food Sci 77:C506–C511

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by an EurAsEC program of The Ministry of Education and Science of the Russian Federation, project No 16.11.M04.0010.

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Authors and Affiliations

  1. G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, 142290, Pushchino, Moscow Region, Russia

    Alexander V. Lisov, Oksana V. Belova, Zhanna I. Andreeva-Kovalevskaya, Zhanna I. Budarina, Alexander A. Solonin, Nataliya G. Vinokurova & Alexey A. Leontievsky

  2. Pushchino State Institute of Life Sciences, 3 Prospekt Nauki, 142290, Pushchino, Moscow Region, Russia

    Alexander A. Solonin & Alexey A. Leontievsky

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  1. Alexander V. Lisov
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  2. Oksana V. Belova
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  3. Zhanna I. Andreeva-Kovalevskaya
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  4. Zhanna I. Budarina
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  5. Alexander A. Solonin
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  6. Nataliya G. Vinokurova
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  7. Alexey A. Leontievsky
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Correspondence to Alexander V. Lisov.

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Lisov, A.V., Belova, O.V., Andreeva-Kovalevskaya, Z.I. et al. Recombinant xylanase from Streptomyces coelicolor Ac-738: characterization and the effect on xylan-containing products. World J Microbiol Biotechnol 30, 801–808 (2014). https://doi.org/10.1007/s11274-013-1480-4

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