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Diversity of the intestinal microbiota in different patterns of feeding infants by Illumina high-throughput sequencing

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Abstract

The gastrointestinal microbiota plays a crucial role in the health and disease of the host through its impact on nutrition. Gut microbial composition is related to different diets, but an association of microbiota with different diets in infant has not yet been shown. In this work, we compared the fecal microbiota of breast-fed (BF) and formula-fed infants (FF). By using Illumina high-throughput sequencing and biochemical analyses, we found differences in gut microbiota between the two groups. BF infants showed a significant enrichment of Actinobacteria and Firmicutes and depletion of Proteobacteria (P < 0.05), the abundance of Bacteroidetes in the two groups was very low (P > 0.05). Enterobacteriaceae (Proteobacteria) were the dominant bacteria in FF infant fecal microbiota, and Veillonellaceae (Firmicutes) and Enterobacteriaceae (Proteobacteria) were the dominant bacteria in the BF infant fecal microbiota. The number of genera (percentage of sequences >0.1 %) in BF and FF infants was 17 and 15 respectively, and Streptococcus was the dominant bacterial genus in both groups.

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Acknowledgments

The authors wish to thank the Program for Changjiang Scholars and Innovative Research Team in Northeast Agricultural University (IRT0959) for funding for this study.

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Authors and Affiliations

  1. Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030, China

    Wenguang Fan, Guicheng Huo, Xiaomin Li, Lijie Yang, Cuicui Duan & Tingting Wang

  2. Henan University of Science and Technology, Luo Yang, 471023, China

    Junliang Chen

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  1. Wenguang Fan
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  2. Guicheng Huo
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  3. Xiaomin Li
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  4. Lijie Yang
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  5. Cuicui Duan
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  7. Junliang Chen
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Correspondence to Guicheng Huo.

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Fan, W., Huo, G., Li, X. et al. Diversity of the intestinal microbiota in different patterns of feeding infants by Illumina high-throughput sequencing. World J Microbiol Biotechnol 29, 2365–2372 (2013). https://doi.org/10.1007/s11274-013-1404-3

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