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Land-use change and soil type are drivers of fungal and archaeal communities in the Pampa biome

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Abstract

The current study aimed to test the hypothesis that both land-use change and soil type are responsible for the major changes in the fungal and archaeal community structure and functioning of the soil microbial community in Brazilian Pampa biome. Soil samples were collected at sites with different land-uses (native grassland, native forest, Eucalyptus and Acacia plantation, soybean and watermelon field) and in a typical toposequence in Pampa biome formed by Paleudult, Albaqualf and alluvial soils. The structure of soil microbial community (archaeal and fungal) was evaluated by ribosomal intergenic spacer analysis and soil functional capabilities were measured by microbial biomass carbon and metabolic quotient. We detected different patterns in microbial community driven by land-use change and soil type, showing that both factors are significant drivers of fungal and archaeal community structure and biomass and microbial activity. Fungal community structure was more affected by land-use and archaeal community was more affected by soil type. Irrespective of the land-use or soil type, a large percentage of operational taxonomic unit were shared among the soils. We accepted the hypothesis that both land-use change and soil type are drivers of archaeal and fungal community structure and soil functional capabilities. Moreover, we also suggest the existence of a soil microbial core.

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Acknowledgments

The authors acknowledge the National Council for Scientific and Technological Development (CNPq - Brazil) and the Coordination for the Improvement of Higher Education Personnel (CAPES - Brazil) for their financial support. This work was supported by the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS process no. 1012030) and by the National Council for Scientific and Technological Development (CNPq—Brazil process no. 476762/2010-30). We also would like to thank the anonymous reviewers for their valuable suggestions and corrections. Special thanks are due to the landholders for granting access to their properties.

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Authors and Affiliations

  1. Departamento de Solos, Universidade Federal de Santa Maria, Av. Roraima 1000, Santa Maria, RS, 97105900, Brazil

    Manoeli Lupatini, Rodrigo Josemar Seminoti Jacques & Zaida Inês Antoniolli

  2. Universidade Federal do Pampa, Campus São Gabriel, Av. Antonio Trilha 1847, São Gabriel, RS, 97300-000, Brazil

    Afnan Khalil Ahmad Suleiman & Luiz Fernando Würdig Roesch

  3. Department of Physical and Environmental Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON, M1C 1A4, Canada

    Roberta R. Fulthorpe

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  1. Manoeli Lupatini
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  2. Rodrigo Josemar Seminoti Jacques
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  4. Afnan Khalil Ahmad Suleiman
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  5. Roberta R. Fulthorpe
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  6. Luiz Fernando Würdig Roesch
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Corresponding author

Correspondence to Luiz Fernando Würdig Roesch.

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Table S1

Soil chemical and physical properties of surface soils (0–5 cm) from twelve points encompassing two sites (A and B) with different land-uses and one site (C) with different soil types from Brazilian Pampa biome (DOCX 20 kb)

Fig. S1

(A) Map of Brazil and of the state of Rio Grande do Sul, showing the location of Rosário do Sul and São Gabriel municipalities and the Pampa bioma. (B) Site A, located in Rosário do Sul. (C) Site B, located in São Gabriel. (D) Site C, located in São Gabriel. The markers indicate points of collections in different land-uses and soil types (TIFF 8547 kb)

Fig. S2

Archaeal community from site A, Pampa biome. APA = Acacia plantation; NGA: Native grassland; SFA = Soybean field; NFA = Native forest; M: GelPilot 100 bp Plus Ladder (Qiagen) (TIFF 4548 kb)

Fig. S3

Fungal community from site A, Pampa biome. APA = Acacia plantation; NGA: Native grassland; SFA = Soybean field; NFA = Native forest; M: GelPilot 100 bp Plus Ladder (Qiagen) (TIFF 4204 kb)

Fig. S4

Archaeal community from site B, Pampa biome. EPB = Eucalyptus plantation; NGB = Native grassland; WFB = Watermelon field; NFB = Native forest; M: GelPilot 100 bp Plus Ladder (Qiagen) (TIFF 4784 kb)

Fig. S5

Fungal community from site B, Pampa biome. EPB = Eucalyptus plantation; NGB = Native grassland; WFB = Watermelon field; NFB = Native forest; M: GelPilot 100 bp Plus Ladder (Qiagen). X: sample not used for analysis (TIFF 3936 kb)

Fig. S6

Archaeal community from site C, Pampa biome. T(1:4)C = Toposequence; M: GelPilot 100 bp Plus Ladder (Qiagen) (TIFF 4153 kb)

Fig. S7

Fungal community from site C, Pampa biome. T(1:4)C = Toposequence; M: GelPilot 100 bp Plus Ladder (Qiagen) (TIFF 4960 kb)

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Lupatini, M., Jacques, R.J.S., Antoniolli, Z.I. et al. Land-use change and soil type are drivers of fungal and archaeal communities in the Pampa biome. World J Microbiol Biotechnol 29, 223–233 (2013). https://doi.org/10.1007/s11274-012-1174-3

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