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Antioxidative properties and inhibitory effect of Bifidobacterium adolescentis on melanogenesis

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Abstract

Melanin is a dark pigment produced by melanocytes. Tyrosinase is a key enzyme which catalyzes the rate-limiting step of melanogenesis. However, accumulation of melanin leads to various skin hyperpigmentation disorders. To find a novel skin-whitening agent, the antioxidant capacity of Bifidobacterium adolescentis culture filtrate and inhibitory effect on melanogenesis were investigated. The antioxidant effects of B. adolescentis culture filtrate include 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)(ABTS) radical cation scavenging activity and reducing power were measured spectrophotometrically. The reducing power is a useful index for the evaluation of potential antioxidants which carry out reduction of ferricyanide to ferrocyanide. Furthermore, the inhibitory effects of the bacterial culture filtrate on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were also determined. The results revealed that B. adolescentis culture filtrate (2.5, 5.0 and 7.5 %; v/v) effectively scavenged DPPH and ABTS radicals, and lower concentrations of the bacterial culture filtrates (0.5, 1.0 and 1.5 %; v/v) showed potent reducing power in a dose-dependent pattern. Additionally, the bacterial culture filtrate suppressed murine tyrosinase activity and decreased the amount of melanin in a dose-dependent manner. Our results demonstrated that B. adolescentis culture filtrate decreases the melanogenesis process of melanoma cells by inhibiting tyrosinase activity, which we suggest may be mediated through its antioxidant activity.

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Acknowledgments

The authors gratefully acknowledge financial support by grants from Ministry of Economic Affairs, ROC. (98-EC-17-A-17-S1-128 and S09900074-603).

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Authors and Affiliations

  1. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan

    Huey-Chun Huang

  2. Department of Applied Cosmetology and Master Program of Cosmetic Science, Hung Kuang University, No. 34, Chung-Chie Rd, Shalu, Taichung County, 43302, Taiwan, ROC

    Tsong-Min Chang

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Correspondence to Tsong-Min Chang.

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Huang, HC., Chang, TM. Antioxidative properties and inhibitory effect of Bifidobacterium adolescentis on melanogenesis. World J Microbiol Biotechnol 28, 2903–2912 (2012). https://doi.org/10.1007/s11274-012-1096-0

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  • DOI: https://doi.org/10.1007/s11274-012-1096-0

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