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Isolation and characterisation of aerobic endospore forming Bacilli from sugarcane rhizosphere for the selection of strains with agriculture potentialities

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Abstract

Eighteen aerobic endospore forming strains were isolated from sugarcane rhizosphere in N-free medium. A phenotypic description and analysis of the 5′ end hypervariable region sequences of 16S rRNA revealed a high diversity of Bacillus and related genera. Isolates were identified, and four genera were obtained: seven strains belonged to Bacillus (Bacillaceae family), four belonged to Paenibacillus, six belonged to Brevibacillus and one strain was identified as Cohnella (Paenibacillaceae family). Four Brevibacillus strains showed in vitro inhibitory activity against plant pathogens fungi Curvularia and Fusarium. Seventy-four percent of the isolated bacteria grew on pectin as the only carbon source, showing polygalacturonase activity. Pectate lyase activity was detected for the first time in a Brevibacillus genus strain. All isolates showed endoglucanase activity. Calcium phosphate solubilisation was positive in 83.3% of the isolates, with higher values than those reported for Bacillus inorganic phosphate solubilising strains. High ethylene plant hormone secretion in the culture medium was detected in 22% of the bacteria. This is the first report of ethylene secretion in Paenibacillaceae isolates. Indole-3-acetic acid production was found in a Brevibacillus genus isolate. It was reported for the first time the presence of Cohnella genus strain on sugarcane rhizosphere bearing plant growth promoting traits. The sugarcane isolate Brevibacillus B65 was identified as a plant growth inoculant because it showed wider spectra of plant stimulation capabilities, including an antifungal effect, extracellular hydrolases secretion, inorganic phosphate solubilisation and plant hormone liberation. In this work, sugarcane was shown to be a suitable niche for finding aerobic endospore forming ‘Bacilli’ with agriculture biotechnological purposes.

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References

  • Alfonso F, Guardia E, Rodríguez J, Alfonso I (1999) Biocontrol de patologías fúngicas en semilla botánica de la caña de azúcar. Centro Agrícola 26(2):5–10

    Google Scholar 

  • Amadi JE (2002) Studies on the Mycoflora of sugarcane (Saccharum officinarum L) seed and seedlings. NISEB J 2(1):089–095

    Google Scholar 

  • Anuradha P, Vijayalakshmi K, Prasanna ND, Sridevi K (2007) Production and properties of alkaline xylanases from Bacillus sp. isolated from sugarcane fields. Curr Sci 92(9):1283–1286

    CAS  Google Scholar 

  • Bae M, Kim MY (1997) A new alkalophilic bacterium producing ethylene. J Microbiol Biotechnol 7(3):212–214

    Google Scholar 

  • Basu S, Ghosh A, Bera A, Saha MN, Chattopadhyay D, Chakrabarti K (2008) Thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate Bacillus pumilus DKS1. Bioresour Techn 99:8088–8094

    Article  CAS  Google Scholar 

  • Beneduzi A, Peres D, Beschoren da Costa P, Bodanese MH, Pereira LM (2008a) Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil. Res Microbiol 159:244–250

    Article  CAS  Google Scholar 

  • Beneduzi A, Peres D, Vargas LK, Bodanese–Zanettini MH, Passaglia LMP (2008b) Evaluation of genetic diversity and plant growth promoting activities of nitrogen–fixing Bacilli isolated from rice fields in South Brazil. Appl Soil Ecol 39:311–320

    Article  Google Scholar 

  • Cakmakci R, Donmez MF, Aydin A, Sahin F (2006) Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biol Biochem 38:1482–1487

    Article  CAS  Google Scholar 

  • Cakmakci R, Donmez MF, Erdogan U (2007) The effect of plant growth promoting rhizobacteria on barley seedling growth, nutrient uptake, some soil properties, and bacterial counts. Turk J Agric For 31:189–199

    CAS  Google Scholar 

  • Chang CH, Yang SS (2009) Thermo-tolerant phosphate—solubilizing microbes for multi-functional biofertilizer preparation. Bioresour Techn 100:1648–1658

    Article  CAS  Google Scholar 

  • Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC (2006) Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol 34:33–41

    Article  Google Scholar 

  • Cohelo MRR, Faria F, Portilho N, Evodio I, Edilson P, Soares A, Sedin L (2007) Diversity of Paenibacillus spp. in the rhizosphere of four sorghum (Sorghum bicolor) cultivars sown with two contrasting levels of nitrogen fertilizer assessed by rpoB-based PCR-DGGE and sequencing analysis. J Microbiol Biotechnol 17(5):753–760

    Google Scholar 

  • Collins CH (1967) Métodos microbiológicos. Editorial Acribia, Zaragoza, España

  • Compant S, Clément C, Sessitsch A (2010) Plant growth-promoting bacteria in the rhizo and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol Biochem 42:669–678

    Article  CAS  Google Scholar 

  • de Lima TC, Grisi BM, Bonato MC (1999) Bacteria isolated from a sugarcane agroecosystem: their potential production of polyhydroxyalcanoates and resistance to antibiotics. Rev Microbiol 30:214–224

    Article  Google Scholar 

  • de Oliveira EJ, Rabinovitch L, Gomes R, Konovaloff L, Zahner V (2004) Molecular Characterization of Brevibacillus laterosporus and its potential use in biological control. Appl Environ Microbiol 70(11):6657–6664

    Article  Google Scholar 

  • do Vale M, Seldin L, de Araújo FF, de Lima R (2010) Plant growth promoting rhizobacteria: fundamentals and applications. In: Maheshwari DK (ed) Plant growth and health promoting bacteria. Springer, Berlin, pp 21–43

    Google Scholar 

  • Dobbelaere S, Croonenborghs A, Thys A, van de Broek A, Vanderleyden J (1999) Phytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat. Plant Soil 212:155–164

    Article  CAS  Google Scholar 

  • Döbereiner J (1980) Forage grasses and grain crops. In: Bergersen FJ (ed) Methods for evaluating biological nitrogen fixation. Wiley, Chichester, pp 535–555

    Google Scholar 

  • Doi RH, McGloughlin M (eds) (1992) Biology of Bacilli: applications to industry. Batherworth—Hirneman, Boston

  • Edwards SG, Seddon B (2001) Mode of antagonism of Brevibacillus brevis against Botrytis cinerea in vitro. J Appl Microbiol 91:652–659

    Article  CAS  Google Scholar 

  • Erturk Y, Ercisli S, Haznedar A, Cakmakci R (2010) Effects of plant growth promoting rhizobacteria (PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings. Biol Res 43:91–98

    Article  Google Scholar 

  • Fernández LA, Zalba P, Gómez MA, Sagardoy MA (2005) Bacterias solubilizadoras de fosfato inorgánico aisladas de suelos de la región sojera. Ci Suelo (Argentina) 23(1):31–37

    Google Scholar 

  • Garbeva P, van Veen JÁ, van Elsas JD (2003) Predominant Bacillus spp. in agricultural soil under different management regimes detected via PCR-DGGE. Microb Ecol 45:302–316

    Article  CAS  Google Scholar 

  • García-Fragile P, Velázquez E, Mateos PF, Martínez-Molina E, Rivas R (2008) Cohnella phaseoli sp. nov., isolated from root nodules of Phaseolus coccineus in Spain, and emended description of the genus Cohnella. Int J Syst Evol Microbiol 58(8):1855–1859

    Article  Google Scholar 

  • Garrity GM, Bell JA, Lilburn TG (2004) Taxonomic outline of the procaryotes. Bergey’s manual of systematic bacteriology, 2nd edn. Release 5.0. Springer, New York

  • Goto K, Omura T, Hara Y, Sadaie Y (2000) Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J Gen Appl Microbiol 46:1–8

    Article  CAS  Google Scholar 

  • Goto K, Kato Y, Asahara M, Yokota Y (2002a) Evaluation of the hypervariable region in the 16S rDNA sequence as an index for rapid species identification in the genus Paenibacillus. J Gen Appl Microbiol 48:281–285

    Article  CAS  Google Scholar 

  • Goto K, Mochida K, Asahara M, Suzuki M, Yokota A (2002b) Application of the hypervariable region of the 16S rDNA sequence as an index for the rapid identification of species in the genus Alicyclobacillus. J Gen Appl Microbiol 48:243–250

    Article  CAS  Google Scholar 

  • Goto K, Fujita R, Kato Y, Asahara M, Yokota A (2004) Reclassification of Brevibacillus brevis strains NCIMB 13288 and DSM 6472 (=NRRL NRS-887) as Aneurinibacillus danicus sp. nov. and Brevibacillus limnophilus sp. nov. Int J Syst Evol Microbiol 54(2):419–427

    Article  CAS  Google Scholar 

  • Gray EJ, Smith DL (2005) Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol Biochem 37:395–412

    Article  CAS  Google Scholar 

  • Grichko VP, Glick BR (2001) Amelioration of flooding stress by ACC deaminase-containing plant growth-promoting bacteria. Plant Physiol Biochem 39:11–17

    Article  CAS  Google Scholar 

  • Hankin L, Zucker M, Sands DC (1971) Improved solid medium for the detection and enumeration of pectolytic bacteria. Appl Microbiol 22(2):205–209

    CAS  Google Scholar 

  • Harma DC, Satyanarayana T (2006) A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. Bioresour Technol 97:727–733

    Article  Google Scholar 

  • Hassan MN, Osborn M, Hafeez FY (2010) Molecular and biochemical characterization of surfactin producing Bacillus species antagonistic to Colletotrichum falcatum Went causing sugarcane red rot. Afr J Microbiol Res 4(20):2137–2142

    CAS  Google Scholar 

  • Hayat R, Ali S, Amara U, Khalid R, Ahmed I (2010) Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol 60:579–598

    Article  Google Scholar 

  • Hungria M, Campos RJ (2006) Inoculantes Microbianos: Situação no Brasil. In: Izaguirre-Mayoral ML, Labandera C, Sanjuan J (eds) Biofertilizantes en Iberoamérica: Una visión técnica, científica y empresarial. Montevideo, pp 22–31

  • Kämpfer P, Rosselló–Mora R, Falsen E, Hans-Jürgen B, Tindall BJ (2006) Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Intern J Syst Evol Microbiol 56:781–786

    Article  Google Scholar 

  • Khan AA, Jilani G, Akhtar MS, Naqvi SMS, Rasheed M (2009) Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J Agric Biol Sci 1(1):48–58

    Google Scholar 

  • Kildea S, Ransbotyn V, Khan MR, Fagan B, Leonard G, Mullins E, Doohan FM (2008) Bacillus megaterium shows potential for the biocontrol of Septoriatritici blotch of wheat. Biol Control 47:37–45

    Article  Google Scholar 

  • Lai WA, Young CC (2006) Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol 34:33–41

    Article  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematic. Wiley, Chichester, pp 115–175

    Google Scholar 

  • Lee BH, Kim BK, Lee YJ, Chung CH, Lee JW (2010) Industrial scale of optimization for the production of carboxymethylcellulase from rice bran by a marine bacterium, Bacillus subtilis subsp. subtilis A-53. Enzyme Microb Technol 46:38–42

    Article  CAS  Google Scholar 

  • Li Y, Solomon S (2003) Ethephon: a versatile growth regulator for sugar cane industry. Sugar Tech 5(4):213–223

    Article  CAS  Google Scholar 

  • Li B, Guan Lin X, Soad A, Coosemads J (2005) Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria. J Zhejiang Univ Sci 68(6):496–501

    Article  Google Scholar 

  • Li YH, Zhu JN, Zhai ZH, Zhang Q (2010) Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China). FEMS Microbiol Lett 309:84–93

    CAS  Google Scholar 

  • Logan NA, Berge O, Bishop AH, Busse HJ, De Vos P, Fritze D, Heyndrickx M, Kämpfer P, Rabinovitch L, Salkinoja-Salonen MS, Seldin L, Ventosa A (2009) Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121

    Article  CAS  Google Scholar 

  • Madhaiyan M, Poonguzhali S, Lee JS, Lee KC, Hari K (2011) Bacillus rhizosphaerae sp. nov., a novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. Antonie Van Leeuwenhoek 100:437–444

    Google Scholar 

  • Magnani GS, Didonet CM, Cruz LM, Picheth CF, Pedrosa FO, Souza EM (2010) Diversity of endophytic bacteria in Brazilian sugarcane. Gen Mol Res 9(1):250–258

    Article  CAS  Google Scholar 

  • Mahalakshmi S, Reetha D (2009) Assessment of plant growth promoting activities of bacterial isolates from the rhizosphere of tomato (Lycopersicon esculentum L.). Recent Res Sci Technol 1(1):026–029

    CAS  Google Scholar 

  • Maki M, Leung KT, Qin W (2009) The prospects of cellulose-producing bacteria for the bioconversion of lignocellulosic biomass. Int J Biol Sci 5:500–516

    Article  CAS  Google Scholar 

  • Mayilraj S, Ruckmani A, Kaur Ch, Kaur I, Klenk H-P (2011) Cohnella ferri sp. nov. A novel member of the genus cohnella isolated from haematite ore. Curr Microbiol 62:1704–1709

    Article  CAS  Google Scholar 

  • McSpadden BB (2004) Ecology of Bacillus and Paenibacillus spp. in Agricultural Systems. Phytopathol 94:1252–1258

    Article  Google Scholar 

  • Medina Basso NL (2009) Presente y futuro de los biofertilizantes en Iberoamérica: Cuba. In: Biofertilizantes para la Agricultura de Iberoamérica en el Siglo XXI. Taller Biofag: Fertilizantes Biológicos para la Agricultura y el Medio Ambiente. Book Abstracts, University of Havana

  • Mehdi SS (2001) Plant growth hormones: growth promoters and inhibitors. In: Pessarakli M (ed) Handbook of plant and crop physiology. Tukson, Arizona

    Google Scholar 

  • Mehta P, Chauhan A, Mahajan R, Mahajan PK, Shirkot CK (2010) Strain of Bacillus circulans isolated from apple rhizosphere showing plant growth promoting potential. Curr Sci 98(4):538–542

    CAS  Google Scholar 

  • Mendes R, Pizzirani-Kleiner AA, Araujo WL, Raaijmakers JM (2007) Diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of Burkholderia cepacia complex isolates. Appl Environ Microbiol 73(22):7259–7267

    Article  CAS  Google Scholar 

  • Min-Ho Y, Ten LN, Wan-Taek IM (2007) Cohnella panacarvi sp. nov., a Xylanolytic bacterium isolated from ginseng cultivating soil. J Microbiol Biotechnol 6:913–918

    Google Scholar 

  • Mirabal LA, Ortega E, Rodés R, Fernández L, Pérez E (2000) Another nitrogen–fixing microorganism in sugarcane stalks: bacillus brevis. Cultiv Tropicales 21(4):9–12

    Google Scholar 

  • Molva C, Sudagidan M, Okuklu B (2009) Extracellular enzyme production and enterotoxigenic gene profiles of Bacillus cereus and Bacillus thuringiensis strains isolated from cheese in Turkey. Food Control 20:829–834

    Article  CAS  Google Scholar 

  • Muhammad SN, Amusa A (2003) In vitro inhibition of growth of some seedling blight inducing pathogens by compost inhabiting microbes. Afr J Biotechnol 2:161–164

    Google Scholar 

  • Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Ann Chim Acta 27:31–36

    Article  CAS  Google Scholar 

  • Nishijima T, Toyota K, Michizuki M (2005) Predominant culturable Bacillus species in japanese arable soils and their potential as biocontrol agents. Microbes Environ 21:61–68

    Article  Google Scholar 

  • Ortíz-Castro R, Valencia–Cantero E, López–Bucio J (2008) Plant growth promotion by Bacillus megaterium involves cytokinin signalling. Plant Sign Beh 3(4):263–265

    Article  Google Scholar 

  • Ouattara HG, Reverchon S, Niamke SL, Nasser W (2010) Molecular identification and pectate lyase production by Bacillus strains involved in cocoa fermentation. Food Microbiol 28(1):1–8

    Article  Google Scholar 

  • Pons TL, Anten NPR (2004) Is plasticity in partitioning of photosynthetic resources between and within leaves important for whole-plant carbon gain in canopies? Funct Ecol 18:802–811

    Article  Google Scholar 

  • Raaijmakers JM, Vlami M, de Souza JT (2002) Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek 81:537–547

    Article  CAS  Google Scholar 

  • Raddadi N, Cherif A, Boudabous A, Daffonchio D (2008) Screening of plant growth promoting traits of Bacillus thuringiensis. Ann Microbiol 58(1):47–52

    Article  CAS  Google Scholar 

  • Rastogi G, Bhalla A, Adhikari A, Bischoff KM, Hughes SR, Christopher LP, Sani RK (2010) Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains. Bioresour Techn 101:8798–8806

    Article  CAS  Google Scholar 

  • Rennie RJ, de Freitas JR, Ruschel AP, Vose PB (1982) Isolation and identification of N2-fixing bacteria associated with sugar cane (Saccharum sp.). Can J Microbiol 28(5):462–467

    Article  CAS  Google Scholar 

  • Reva ON, Dixelius C, Meijer J, Priest FG (2004) Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis. FEMS Microbiol Ecol 48:249–259

    Article  CAS  Google Scholar 

  • Rivas R, García-Fraile P, Zurdo-Piñeiro JL, Mateos PF, Martínez Molina E, Bedmar EJ, Sánchez-Raya J, Velázquez E (2008) Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane. Int J Syst Evol Microbiol 58:1850–1854

    Article  CAS  Google Scholar 

  • Rodríguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotech Adv 17:319–339

    Article  Google Scholar 

  • Rojas A, Rodríguez AM, Montes S, Pérez S (2010) Evaluación de la promoción del crecimiento de Cynodon dactylon L. por rizobacterias productoras de fitohormonas aisladas de un suelo contaminado con hidrocarburos derivados del petróleo. Polibotánica 29:131–147

    Google Scholar 

  • Saidi N, Kouki SM, Hiri F, Hajlaoui MR, Mahrouk M, Ouzari H, Jedidi N, Hassen A (2009) Characterization and selection of Bacillus sp. strains, effective biocontrol agents against Fusarium oxysporum f. sp. Radicis—lycopersici, the causal agent of Fusarium crown and root rot in tomato. Ann Microbiol 59(2):191–198

    Article  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1987) Molecular cloning–a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Shida O, Takagi H, Kadowaki K, Nakamura LK, Komagata K (1996) Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946

    Article  CAS  Google Scholar 

  • Soriano M, Díaz P, Pastor FIJ (2005) Pectinolytic systems of two aerobic sporogenous bacterial strains with high activity on pectin. Curr Microbiol 50:114–118

    Article  CAS  Google Scholar 

  • Swain MR, Ray RC (2009) Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora. Microbiol Res 164:121–130

    Article  CAS  Google Scholar 

  • Tabao NSC, Monsalud RG (2010) Screening and optimization of cellulase production of Bacillus strains isolated from philippine mangroves. Philippine J Syst Biol IV:79–87

    Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  Google Scholar 

  • Teng JL, Woo PC, Leung KW, Lau SK, Wong MK, Yuen KY (2003) Pseudobacteraemia in a patient with neutropenic fever caused by a novel Paenibacillus species: Paenibacillus hongkongensis sp. nov. Mol Pathol 56:29–35

    Article  CAS  Google Scholar 

  • Thuler DS, Floh EIS, Handro W, Barbosa HR (2003) Plant growth regulators and amino acids released by Azospirillum sp. in chemically defined media. Lett Appl Microbiol 37:174–178

    Article  CAS  Google Scholar 

  • Timmusk S, Wagner EGH (1999) The plant-growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: A possible connection between biotic and abiotic stress responses. Mol Plant Microbe Int 12(11):951–959

    Article  CAS  Google Scholar 

  • Upadhyay SK, Singh DP, Saikia R (2009) Genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition. Curr Microbiol 59(5):489–496

    Article  CAS  Google Scholar 

  • Vázquez P, Holguin G, Puente ME, López-Cortes L, Bashan Y (2009) Phosphate—solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol Fertil Soils 30:460–468

    Article  Google Scholar 

  • Wang S, Huijun W, Junqing Q, Lingli M, Jun L, Yanfei X, Xuewen G (2009) Molecular mechanism of plant growth promotion and induced systemic resistance to tobacco mosaic virus by Bacillus spp. J Microbiol Biotechnol 19(10):1250–1258

    Article  CAS  Google Scholar 

  • Yang JH, Liu HX, Zhu GM, Pan JL, Xu LP, Guo JH (2008) Diversity analysis of antagonists from rice-associated bacteria and their application in biocontrol of rice diseases. J Appl Microbiol 104:91–104

    CAS  Google Scholar 

  • Zhang X, Zhang B, Zhang Z, Shen W, Yang C, Yu J, Zhao Y (2005) Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field. J Zhejiang Univ Sci 6B(8):770–777

    Article  Google Scholar 

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Acknowledgments

The authors wish to thank the program of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior do Ministério da Educação (Brazil), and the Ministerio de Educación Superior (Cuba) for grants and fellowships; Fazenda São Francisco for kindly providing the sugarcane samples; and Dr. Felipe Ibañez de Santi Ferrara for volunteering his technical and linguistic skills for the English version of this paper.

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  1. Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente, Av. Patricio Lumumba S/N, 90500, Santiago de Cuba, Cuba

    Teresa de los Milagros Orberá Ratón, Odalys Rodríguez Gámez & Manuel de Jesús Serrat Díaz

  2. Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, São Paulo, SP, 05508-900, Brazil

    Ricardo Yano & Heloíza Ramos Barbosa

  3. Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, Brazil

    Eny Iochevet Segal Floh

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de los Milagros Orberá Ratón, T., Yano, R., Rodríguez Gámez, O. et al. Isolation and characterisation of aerobic endospore forming Bacilli from sugarcane rhizosphere for the selection of strains with agriculture potentialities. World J Microbiol Biotechnol 28, 1593–1603 (2012). https://doi.org/10.1007/s11274-011-0965-2

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