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Screening for glycosidase activities of lactic acid bacteria as a biotechnological tool in oenology

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Abstract

The aim of this study was to evaluate the ability from a number of lactic acid bacteria isolated from different sources to produce glycosidase enzymes. Representative isolates (225) from clusters obtained after genotyping, using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis, of 1,464 isolates, were screened for β-D-glucosidase activity. Thirty-five of them were selected for subsequent analysis. These strains were able to hydrolyze α-D-glucopyranoside, β-D-xylopyranoside and α-L-arabinofuranoside although β-D-glucosidase activity was the predominant activity for 22 of the selected strains. Only some of them did so with α-L-rhamnopyranoside. All of these were from wine samples and were identified as belonging to the Oenococcus oeni species using Amplification and Restriction Analysis of 16S-rRNA gene (16S-ARDRA). When the influence of pH, temperature and ethanol or sugars content on β-D-glucosidase activity was assayed, a strain-dependent response was observed. The β-D-glucosidase activity occurred in both whole and sonicated cells but not in the supernatants from cultures or obtained after cell sonication. Strains 10, 17, 21, and 23 retained the most β-D-glucosidase activity when they were assayed at the conditions of temperature, pH, ethanol and sugar content used in winemaking. These results suggest that these strains could be used as a source of glycosidase enzymes for use in winemaking.

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Acknowledgments

The authors wish to thank Consejería de Educación y Ciencia of the Council of Communities of Castilla-La Mancha (JCCM) for the project POII09-0159-1914. F. Pérez-Martín is supported by a grant of the Council of Communities of Castilla-La Mancha. P. M. Izquierdo acknowledges the Fondo Social Europeo and INCRECYT for cofounding his contract.

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Authors and Affiliations

  1. Department of Analytical Chemistry and Food Technology, Environmental Sciences and Biochemistry Faculty, University of Castilla-La Mancha, Avda. Carlos III, s/n, 45071, Toledo, Spain

    Fátima Pérez-Martín, Susana Seseña & María Llanos Palop

  2. Institute of Vine and Wine of Castilla-La Mancha, Crta. Toledo-Albacete, s/n, 13700, Tomelloso Ciudad Real, Spain

    Pedro Miguel Izquierdo

  3. Department of Mathematics, Statistics and Operational Research, Institute of Applied Mathematics in Science and Engineering, School of Architecture, University of Castilla-La Mancha, Avda. Carlos III s/n, 45071, Toledo, Spain

    Raúl Martín

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  1. Fátima Pérez-Martín
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  2. Susana Seseña
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  3. Pedro Miguel Izquierdo
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  5. María Llanos Palop
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Correspondence to Susana Seseña.

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Pérez-Martín, F., Seseña, S., Izquierdo, P.M. et al. Screening for glycosidase activities of lactic acid bacteria as a biotechnological tool in oenology. World J Microbiol Biotechnol 28, 1423–1432 (2012). https://doi.org/10.1007/s11274-011-0942-9

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