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Enhancement of diepoxin ζ production in liquid culture of endophytic fungus Berkleasmium sp. Dzf12 by polysaccharides from its host plant Dioscorea zingiberensis

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Abstract

This study is the first report of the enhancement of diepoxin ζ production in liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 by the polysaccharides from its host plant Dioscorea zingiberensis which serve as elicitors. Three polysaccharides, namely water-extracted polysaccharide (WEP), sodium hydroxide-extracted polysaccharide and acid-extracted polysaccharide were sequentially prepared from the rhizomes of D. zingiberensis. Among them, WEP was found to be the most effective elicitor to enhance diepoxin ζ production. When WEP was added to the medium at 400 mg l−1 on day 3 of culture, the maximal diepoxin ζ yield (intracellular diepoxin ζ in mycelia plus extracellular diepoxin ζ in medium) of 350.76 mg l−1 on day 15 was achieved, which was about 2.69-fold in comparison with that (130.43 mg l−1) of the control.

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Acknowledgments

This work was co-financed by the grants from the National Natural Science Foundation of China (31071710 and 30871662), the Natural Science Foundation of Beijing (6092015), and the Program for Changjiang Scholars and Innovative Research Team in University of China (IRT1042).

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Authors and Affiliations

  1. College of Biology and Science, Sichuan Agricultural University, Yaan, 625014, China

    Yan Li & Chunbang Ding

  2. Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China

    Yan Li, Peiqin Li, Yan Mou, Jianglin Zhao, Tijiang Shan & Ligang Zhou

Authors
  1. Yan Li
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  2. Peiqin Li
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  3. Yan Mou
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  4. Jianglin Zhao
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  5. Tijiang Shan
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  6. Chunbang Ding
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  7. Ligang Zhou
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Correspondence to Ligang Zhou.

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Li, Y., Li, P., Mou, Y. et al. Enhancement of diepoxin ζ production in liquid culture of endophytic fungus Berkleasmium sp. Dzf12 by polysaccharides from its host plant Dioscorea zingiberensis . World J Microbiol Biotechnol 28, 1407–1413 (2012). https://doi.org/10.1007/s11274-011-0940-y

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