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A novel β-glucosidase from Aspergillus fumigates releases diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW)

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Abstract

A β-glucosidase effectively releasing diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW), named AfG, was purified from a strain of Aspergillus fumigates. The molecular weight of AfG was 113 kDa. Analysis of protein fragments by ESI-Q-TOF indicated that AfG was a β-glucosidase. The circular dichroism spectrum suggested that the main secondary structure of AfG in Milli-Q water was α-helixes. Atomic force microscopy revealed that it was a globular protein. AfG maintained high activity from pH 3.6 to 5.0 and from 50 to 90°C. With the strong heat stability, AfG retained 55% of its original activity at 65°C for 120 h. AfG utilized muti-3-O-glycosides of various steroidal saponins from DZW as substrate, such as trillin, diosgenin diglucoside, dioscin, deltonin and gracillin, to yield diosgenin, suggesting the possibility of producing diosgenin from total saponins of DZW using a single enzyme.

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Acknowledgments

This work was financially supported by the National “11th-5-Year” and “12th-5-Year” Plan Project of China (2006BAB04A14 and 2011BAJ07B04)” and the National Natural Science Foundation of China (No. 20972105). We would like to thank Professor Iain Bruce from Zhejiang University for his kind help in English editing.

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Authors and Affiliations

  1. Department of Environmental Engineering, Key Laboratory of Water and Sediment Sciences, Ministry of Education, Peking University, Beijing, 100871, China

    Jing Lei & Tianhong Li

  2. Mathematical College, Sichuan University, Chengdu, 610064, China

    Hai Niu

  3. Laboratory of Ethnopharmacology, Regenerative Medicine Research Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, China

    Wen Huang

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  1. Jing Lei
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  2. Hai Niu
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  3. Tianhong Li
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  4. Wen Huang
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Corresponding author

Correspondence to Wen Huang.

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Jing Lei and Hai Niu are contributed equally to this work.

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Lei, J., Niu, H., Li, T. et al. A novel β-glucosidase from Aspergillus fumigates releases diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW). World J Microbiol Biotechnol 28, 1309–1314 (2012). https://doi.org/10.1007/s11274-011-0907-z

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