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In vitro fermentation of prebiotics by Lactobacillus plantarum CFR 2194: selectivity, viability and effect of metabolites on β-glucuronidase activity

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Abstract

Prebiotic Fructooligosaccharides (FOS) escape metabolism in upper GI tract undergo microbial metabolism in colon and thereby influence the nature, type and number of intestinal microbiota to improve host’s health. The present study focuses on the ability of Lactobacillus plantarum CFR 2194 to utilize FOS as a selective carbon and energy source. The effect of fermentative metabolites of L. plantarum on the β-glucuronidase was also investigated. A total of 16 strains of lactobacilli were assessed for their ability to ferment oligosaccharides. L. plantarum CFR 2194, an isolate from kanjika was found to utilize FOS effectively. Lactic acid was the main metabolic end product, followed by acetic acid, butyric acid, formic acid and ethanol. The inhibitory effects of these metabolites have been confirmed through the reduction of β-glucuronidase activity. L. plantarum when co-cultured with β-glucuronidase producing E. coli, in a basal media containing FOS as an energy source, could inhibit the growth of the pathogen during the course of fermentation. The results showed that L. plantarum CFR 2194 has the ability to utilize the prebiotic FOS as a selective carbon and energy source. The organism could inhibit the growth of the pathogen which produces β-glucuronidase and lowered its activity by the metabolites of FOS which indicates the probable use of L. plantarum through dietary intervention in combating colon carcinogenesis.

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Acknowledgments

A.N. Madhu is thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, India for the award of Senior Research Fellowship. The authors thank Director, CFTRI, for supporting the work.

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  1. Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570 020, India

    Madhu Arenahalli Ningegowda & Prapulla Siddalingaiya Gurudutt

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  1. Madhu Arenahalli Ningegowda
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  2. Prapulla Siddalingaiya Gurudutt
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Correspondence to Prapulla Siddalingaiya Gurudutt.

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Arenahalli Ningegowda, M., Siddalingaiya Gurudutt, P. In vitro fermentation of prebiotics by Lactobacillus plantarum CFR 2194: selectivity, viability and effect of metabolites on β-glucuronidase activity. World J Microbiol Biotechnol 28, 901–908 (2012). https://doi.org/10.1007/s11274-011-0887-z

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  • DOI: https://doi.org/10.1007/s11274-011-0887-z

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