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Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain

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Abstract

A new actinomycete strain designated TN256, producing antimicrobial activity against pathogenic bacteria and fungi, was isolated from a Tunisian Saharan soil. Morphological and chemical studies indicated that strain TN256 belonged to the genus Streptomyces. Analysis of the 16S rDNA sequence of strain TN256 showed a similarity level ranging between 99.79 and 97.8% within Streptomyces microflavus DSM 40331T and Streptomyces griseorubiginosus DSM 40469T respectively. The comparison of its physiological characteristics showed significant differences with the nearest species. Combined analysis of the 16 S rRNA gene sequences (FN687758), fatty acids profile, and results of physiological and biochemical tests indicated that there were genotypic and phenotypic differentiations of that isolate from other Streptomyces species neighbours. These date strongly suggest that strain TN256 represents a novel species with the type strain Streptomyces TN256 (=CTM50228T). Experimental validation by DNA–DNA hybridization would be required for conclusive confirmation. Four active products (1–4) were isolated from the culture broth of Streptomyces TN256 using various separation and purification steps and procedures. 1: N-[2-(1H-indol-3-yl)-2 oxo-ethyl] acetamide ‘alkaloid’ derivative; 2: di-(2-ethylhexyl) phthalate, a phthalate derivative; 3: 1-Nonadecene and 4: Cyclo (l-Pro-l-Tyr) a diketopiperazine ‘DKP’ derivative. The chemical structure of these four active compounds was established on the basis of spectroscopic studies NMR and by comparing with data from the literature. According to our biological studies, we showed in this work that the pure compounds (1–4) possess antibacterial and antifungal activities.

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Acknowledgments

This work was supported by the CMCU project No: 06/S 0901 “MELLOULI/AIGLE” 2006–2009.

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Authors and Affiliations

  1. Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, P.B. 1177, 3018, Sfax, Tunisie

    S. Smaoui, L. Elleuch, I. Karray-Rebai & L. Mellouli

  2. Département of Bioprocédés and Systèmes Microbiens, INP-ENSAT, Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), 1 Av, de l’Agrobiopôle, BP 32607, 31326, Castanet-Tolosan, France

    F. Mathieu

  3. Laboratoire de Chimie de Coordination UPR8241 (CNRS), Université de Toulouse, 205 route de Narbonne, 31077, Toulouse cedex 4, France

    Y. Coppel

  4. UMR 5245 CNRS/INP/UPS, EcoLab (Laboratoire d’écologie fonctionnelle), Toulouse, France

    G. Merlina

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  1. S. Smaoui
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  2. F. Mathieu
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  6. I. Karray-Rebai
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  7. L. Mellouli
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Correspondence to L. Mellouli.

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Smaoui, S., Mathieu, F., Elleuch, L. et al. Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain. World J Microbiol Biotechnol 28, 793–804 (2012). https://doi.org/10.1007/s11274-011-0872-6

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  • DOI: https://doi.org/10.1007/s11274-011-0872-6

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