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Ethanol production from sweet sorghum juice in repeated-batch fermentation by Saccharomyces cerevisiae immobilized on corncob

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Abstract

Ethanol fermentation from sweet sorghum juice containing 240 g/l of total sugar by Saccharomyces cerevisiae TISTR 5048 and S. cerevisiae NP 01 immobilized on low-cost support materials, corncob pieces, was investigated. In batch fermentation, S. cerevisiae TISTR 5048 immobilized on 6 × 6 × 6 mm3 corncobs gave higher ethanol production than those immobilized on 12 × 12 × 12 mm3 corncobs in terms of ethanol concentration (P), yield (Y p/s ) and productivity (Q p ) with the values of 102.39 ± 1.11 g/l, 0.48 ± 0.01 and 2.13 ± 0.02 g/l h, respectively. In repeated-batch fermentation, the yeasts immobilized on the 6 × 6 × 6 mm3 corncobs could be used at least eight successive cycles with the average P, Y p/s and Q p of 97.19 ± 5.02 g/l, 0.48 ± 0.02 and 2.02 ± 0.11 g/l h, respectively. Under the same immobilization and repeated-batch fermentation conditions, P (90.75 ± 3.05 g/l) and Q p (1.89 ± 0.06 g/l h) obtained from S. cerevisiae NP 01 were significantly lower than those from S. cerevisiae TISTR 5048 (P < 0.05), while Y p/s from both strains were not different. S. cerevisiae TISTR 5048 immobilized on the corncobs also gave significantly higher P, Y p/s and Q p than those immobilized on calcium alginate beads (P < 0.05).

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Acknowledgments

This research was financially supported by the National Research Council of Thailand (NRCT), Thailand. We would like to thank Assistant Prof. Dr. Paiboon Danviruthai, Faculty of Technology, Khon Kaen University (KKU) for providing the NP 01 strain, Associate Prof. Dr. Prasit Jaisil, Faculty of Agriculture, KKU for providing sweet sorghum juice, and Associate Prof. Dr. Aroonwadee Chanawong, Faculty of Associated Medical Sciences, KKU and Dr. Preekamol Klanrit, Faculty of Technology, KKU for their internal reviews of this paper and helpful suggestions. We also thank to our students, Mr. Suttipong Sak-Ubol, Ms. Sucheera Louwcharoen, Ms. Amornrat Siripan, Mr. Aroon Chanamarn and Mr. Pongthep Ariyajarearnwong for their technical assistance.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand

    Lakkana Laopaiboon & Pattana Laopaiboon

  2. Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen, 40002, Thailand

    Lakkana Laopaiboon

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  1. Lakkana Laopaiboon
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  2. Pattana Laopaiboon
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Correspondence to Pattana Laopaiboon.

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Laopaiboon, L., Laopaiboon, P. Ethanol production from sweet sorghum juice in repeated-batch fermentation by Saccharomyces cerevisiae immobilized on corncob. World J Microbiol Biotechnol 28, 559–566 (2012). https://doi.org/10.1007/s11274-011-0848-6

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  • DOI: https://doi.org/10.1007/s11274-011-0848-6

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