Abstract
The aim of this study was to determine the impact of storage, animal diet, and animal source on the bacterial community composition of manure. The differences among bacterial community structures in fresh manure from cows on two different diets, cow manure stored in a deep pit for about one month, and fresh pig manure were compared. A molecular approach consisting of terminal restriction fragment length polymorphism (T-RFLP), in combination with sequence information from clone libraries, facilitated the identification of specific dominant bacterial populations that varied significantly among manures from different sources and treatments. One such population, represented by TRF 157, the most dominant peak of the bacterial community from stored manure, was identified as a Spirochaeta sp. Interestingly, this peak was absent in the fresh manure communities. The prevailing species in the fresh manure bacterial communities were distinct from those in manure from the storage pit, indicating a major shift in bacterial community composition induced by storage conditions. Moreover, distinct differences in bacterial communities were observed among animal source, but not animal feed. Manure storage is consequently an important parameter to consider when handling fertilizers, in order to obtain an optimal soil microbial ecosystem functioning.
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Arthurson V (2009) Closing the global energy and nutrient cycles through application of biogas residue to agricultural land—potential benefits and drawback. Energies 2(2):226–242
Buret A, den Hollander N, Wallis PM, Befus D, Olson ME (1990) Zoonotic potential of Giardiasis in domestic ruminants. J Infect Dis 162:231–237
Burton CH, Turner C (2003) Manure management. Treatment strategies for sustainable agriculture, 2nd edn. Silsoe Research Institute, Bedford
Canale-Parola E (1980) Revival of the names Spirochaeta litoralis, Spirochaeta zuelzerae, and Spirochaeta aurantia. Int J Syst Bact 30:594
Chapman PA, Siddons CA, Cerman Malo AT, Harkin MA (1997) A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry. Epidemiol Infect 119:245–250
Cotta MA, Whitehead TR, Zeltwanger RL (2003) Isolation, characterization and comparison of bacteria from swine faeces and manure storage pits. Environ Microbiol 5(9):737–745
Davis RD, Carrington EG, Aitken MN, Fenlon DR, Svoboda I (1999) A user’s guide to research on application of organic wastes to land. Report number 11523-0, 1-129. Scotland and Northern Ireland Forum for Environmental Research, Marlow
Farzan A, Friendship RM, Cook A, Pollari F (2009) Occurrence of Salmonella, Campylobacter, Yersinia enterocolitica, Escherichia coli O157 and Listeria monocytogenes in swine. Zoonoses and public health
Hao X, Priya SM, Shah MA, Travis GR (2005) Influence of canola and sunflower diet amendments on cattle feedlot manure. J Environ Qual 34:1439–1445
Himathongkham S, Riemann H (1999) Destruction of Salmonella typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes in chicken manure by drying and/or gassing with ammonia. FEMS Microbiol Lett 171:179–182
Himathongkham S, Bahari S, Riemann H, Cliver DO (1999) Survival of Escherichia coli O157:H7 and Salmonella typhimurium in cow manure and cow manure slurry. FEMS Microbiol Lett 178:251–257
Ibekwe AM, Grieve CM, Lyon SR (2003) Characterization of microbial communities and composition in constructed dairy wetland wastewater effluent. Appl Environ Microbiol 69(9):5060–5069
Ingraham J, Maaloe L, Neidhardt FC (1983) Growth of the bacterial cell. Sinauer Associates Inc, Sunderland
Jacobson LH, Nagle TA, Gregory NG, Bell RG, Roux GL, Haines JM (2002) Effect of feeding pasture-finished cattle different conserved forages on Escherichia coli in the rumen and faces. Meat Sci 62:93–106
Jones PW (1976) The effect of temperature, solids content and pH on the survival of Salmonellas in cattle slurry. British Vet J 132:284–293
Jones K, Howard S, Wallace JS (1999) Intermittent shedding of thermophilic Campylobacters by sheep at pasture. J Appl Microbiol 86:531–536
Kearney TE, Larkin MJ, Levett PN (1993) The effect of slurry storage and anaerobic digestion on survival of pathogenic bacteria. J Appl Microbiol 74:86–93
Kowalchuk GA, Naoumenko ZS, Derikx PJL, Felske A, Stephen JR, Arkhipchenko IA (1999) Molecular analysis of ammonia-oxidizing bacteria of the B subdivision of the class Proteobacteria in compost and composted materials. Appl Environ Microbiol 65(2):396–403
Kudva IT, Hunt CW, Williams CJ, Nance UM, Hovde CJ (1997) Evaluation of dietary influences on Escherichia coli O157:H7 shedding by sheep. Appl Environ Microbiol 63:3878–3886
Kudva IT, Blanch K, Hovde CJ (1998) Analysis of Escherichia coli O157:H7 in ovine or bovine manure and manure slurry. Appl Environ Microbiol 64:3166–3174
Leung K, Topp E (2001) Bacterial community dynamics in liquid swine manure during storage: molecular analysis using DGGE/PCR of 16S rDNA. FEMS Microbiol Ecol 38:169–177
Marti R, Dabert P, Pourcher AM (2009) Pig manure contamination marker selection based on the influence of biological treatment on the dominant fecal microbial groups. Appl Environ Microbiol 75(15):4967–4974
McDermid AS, McKee AS, Marsh PD (1988) Effect of environmental pH on enzyme activity and growth of Bacteroides gingivalis W50. Infect Immun 56(5):1096–1100
McGee DA, Nielsen MK, Rasby RJ, Mader TL (2008) Effects of summer climatic conditions on body temperature in beef cows. Nebraska beef cattle reports, University of Nebraska, Lincoln
McGuirk SM (2002) Managing clostridial diseases in cattle. In: Proceedings of the 2002 midwest dairy herd health conference. University of Wisconsin School of Veterinary Medicine.
Morrison FB (1956) Feeds and feeding. The Morrison Publishing Co, Ithaca
Muyzer G, Teske A, Wirsen CO, Jannasch HW (1995) Phylogenetic relationship of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments. Arch Microbiol 164:162–172
Nicholson FA, Groves SJ, Chambers BJ (2003) Pathogen survival during livestock manure storage and following land application. Bioresour Technol 96:135–143
Odlare M, Pell M, Svensson K (2008) Changes in soil chemical and microbiological properties during 4 years of application of various organic residues. Waste Manag 28:1246–1253
Peu P, Brugere H, Pourcher AM, Kerouredan M, Godon JJ, Delgenes JP, Dabert P (2006) Dynamics of a pig slurry microbial community during anaerobic storage and management. Appl Environ Microbiol 72(5):3578–3585
Spoelstra SF (1978) Enumeration and isolation of anaerobic microbiota of piggery wastes. Appl Environ Microbiol 35:841–846
Spoelstra SF (1980) Origin of objectionable odorous components in piggery wastes and the possibility of applying indicator components for studying odor development. Agric Environ 5:241–260
Spörndly R (1999) Fodertabeller för idisslare. Swedish Univeristy of Agricultural Sciences, Uppsala
Stanley KN, Wallace JS, Jones K (1998) Thermophilic Campylobacters in dairy slurries on Lancashire farms: seasonal effects of storage and land application. J Appl Microbiol 85:405–409
Trochimchuk T, Fotheringham J, Topp E, Schraft H, Leung KT (2003) A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure. J Microbiol Methods 54(2):165–175
Wang G, Zhao T, Doyle MP (1996) Fate of enterohemorrhagic Escherichia coli O157:H7 in bovine feces. Appl Environ Microbiol 62:2567–2570
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bact 173(2):697–703
Whitehead TR, Cotta MA (2001) Characterisation and comparison of microbial populations in swine faeces and manure storage pits by 16S rDNA gene sequences analyses. Anaerobe 7:181–187
Yashurar A, Fuwa K, Jimbu M (1984) Identification of odorous compounds in fresh and rotten swine manure. Agric Biol Chem 48:3001–3010
Zhang RH, Day DL (1996) Anaerobic decomposition of swine manure and ammonia generation in a deep pit. Trans ASAE 39(5):1811–1815
Zhao T, Doyle MP, Shere J, Garber L (1995) Prevalence of enterohemorrhagic Escherichia coli O157:H7 in a survey of dairy herds. Appl Environ Microbiol 61:1290–1293
Zhu J (2000) A review of microbiology in swine manure odor control. Agric Ecosyst Environ 78:93–106
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This work was supported by grants from the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS).
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Arthurson, V. Storage conditions and animal source influence the dominant bacterial community composition in animal manure. World J Microbiol Biotechnol 27, 2013–2022 (2011). https://doi.org/10.1007/s11274-011-0663-0
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DOI: https://doi.org/10.1007/s11274-011-0663-0