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Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis

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Abstract

The transfer of dibenzothiophene (DBT) and its derivatives into cells is a critical step for biodesulfurization. The desulfurization reactions of resting cells and cell lysate were studied, which showed that the desulfurization rate of DBT, especially 4, 6-dimethyldibenzothiophene (4, 6-DMDBT) in Rhodococcus erythropolis LSSE8-1 was seriously affected by the transfer into cells. The inhibited effect of NaN3 on desulfurization reactions was studied, which confirmed that the transfer of DBT into cells was an active transport in R. erythropolis LSSE8-1. The uptake-genes of DBT and its derivatives (HcuABC) of Pseudomonas delafieldii R-8 were introduced into the specific desulfurization bacterium, R. erythropolis LSSE8-1. Compared with the wild type, the strains bearing HcuABC genes showed a higher desulfurization activity. The desulfurization ratio of DBT showed a 19% increase, and 13% increase of 4, 6-DMDBT.

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Acknowledgments

We thank Warren A. Dick (Centre for Land and Biological Resources Research, Research Branch, Agriculture Canada, Ottawa, Canada) for the gift of the plasmid pBS305. This work was supported by National Natural Science Foundation of China (No. 21076216 and No. 30970046).

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100080, Beijing, China

    Ze-long Wang, Dan Wang, Qiang Li, Wang-liang Li, Huang Tang & Jian-min Xing

  2. Graduate University of Chinese Academy of Sciences, 100039, Beijing, China

    Ze-long Wang, Dan Wang & Huang Tang

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  1. Ze-long Wang
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  2. Dan Wang
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  3. Qiang Li
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  4. Wang-liang Li
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  5. Huang Tang
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  6. Jian-min Xing
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Correspondence to Jian-min Xing.

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Wang, Zl., Wang, D., Li, Q. et al. Enhanced biodesulfurization by expression of dibenzothiophene uptake genes in Rhodococcus erythropolis . World J Microbiol Biotechnol 27, 1965–1970 (2011). https://doi.org/10.1007/s11274-011-0656-z

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  • DOI: https://doi.org/10.1007/s11274-011-0656-z

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