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Molecular differentiation of nocturnally periodic and diurnally sub-periodic Wuchereria bancrofti by Randomly Amplified Polymorphic DNA (RAPD)

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Abstract

Wuchereria bancrofti, a nematode parasite, is responsible for causing 90% of lymphatic filariasis infection in the world. In India, W. bancrofti exists in two physiological forms, nocturnally periodic in the main land and diurnally sub-periodic in Car Nicobar group of islands. Differentiation of these two parasitic forms by conventional microscopic methods difficult requiring good skill and hence tedious. Therefore, we developed a simple and rapid Random Amplified Polymorphic DNA (RAPD) assay to differentiate these parasitic forms. Also, the phylogenetic relationship between periodic and sub-periodic W. bancrofti and also Brugia malayi populations was analyzed using RAPD profile generated. Distinct RAPD profiles were observed among the three parasites with the formation of three distinct clusters in the phylogenetic tree. Substantial genetic diversity (Nei’s genetic diversity H) was observed among periodic (H = 0.0577) and sub-periodic (H = 0.1415) W. bancrofti populations.

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References

  • Bandi C, La Rosa G, Comincini S, Damiani G, Pozio E (1993) Random amplified polymorphic DNA technique for the identification of Trichinella species. Parasitology 107:419–424

    Article  CAS  Google Scholar 

  • Basu PC (1958) A note on malaria and filariasis in Andaman and Nicobar. Bull Nat Soc India Mal Mosq Dis 6:193–206

    Google Scholar 

  • Bisht R, Hoti SL, Thangadurai R, Das PK (2006) Isolation of Wuchereria bancrofti microfilariae from archived stained blood slides for use in genetic studies and amplification of parasite and endosymbiont genes. Acta Trop 99(1):1–5

    Article  CAS  Google Scholar 

  • Chow CY (1973) Filariasis vectors in the Western Pacific region. Z Tropenmed Parasitol 24(4):404–418

    CAS  Google Scholar 

  • Dhamodharan R, Das MK, Hoti SL, Das PK, Dash AP (2008) Genetic variability of diurnally sub-periodic Wuchereria bancrofti in Nicobarese tribe of Nicobar group of Islands, Andaman and Nicobar Islands, India. Parasitol Res 103(1):59–66

    Article  CAS  Google Scholar 

  • Dondero TJ Jr, Sivanandam S (1971) Diurnally sub-periodic microfilarial pattern in Brugia malayi in West Malaysia. Trans R Soc Trop Med Hyg 65(5):691–693

    Article  Google Scholar 

  • Gasser RB, Hung GC, Chilton NB, Beveridge I (2004) Advances in developing molecular-diagnostic tools for strongyloid nematodes of equids: fundamental and applied implications. Mol Cell Probes 18(1):3–16

    Article  CAS  Google Scholar 

  • Grove DI (1983) Selective primary health care: strategies for the control of disease in the developing world. VII. Filariasis. Rev Infect Dis 5(5):933–944

    Article  CAS  Google Scholar 

  • Hawking F, Denham DA (1976) The distribution of human filariasis throughout the world. Part I. the Pacific Region, including New Guinea. Trop Dis Bull 73(5):347–373

    CAS  Google Scholar 

  • Hoti SL, Subramaniyan K, Das PK (2003) Detection of codon for amino acid 200 in isotype 1 beta-tubulin gene of Wuchereria bancrofti isolates, implicated in resistance to benzimidazoles in other nematodes. Acta Trop 88(1):77–81

    Article  CAS  Google Scholar 

  • Hoti SL, Sharma Rohit, Athisaya Mary K, Dhamodharan R, Krishnamoorthy K, Das PK (2008) A method for detecting microfilaraemia, filarial specific antigens and antibodies and typing of parasites for drug resistance and genotypes using finger prick blood sample. Acta Trop 107(3):268–271

    Article  CAS  Google Scholar 

  • Kalra NL (1974) Filariasis among aborigines of Andaman and Nicobar islands. J Commun Dis 6:40–56

    Google Scholar 

  • Lescuyer P, Picot S, Bracchi V, Burnod J, Austin J, Perard A, Ambroise-Thomas P (1997) Detection of RAPD markers correlated with chloroquine resistance in Plasmodium falciparum. Genome Res 7:747–753

    CAS  Google Scholar 

  • Nei M (1978) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70(12):3321–3323

    Article  Google Scholar 

  • Nuchprayoon S, Junpee A, Poovorawan Y (2007) Random amplified polymorphic DNA (RAPD) for differentiation between Thai and Myanmar strains of Wuchereria bancrofti. Filaria J 6:6

    Article  Google Scholar 

  • Ottesen EA, Ismail MM, Horton J (1999) The Role of albendazole to eliminate lymphatic filariasis. Parasitol Today 15(9):382–386

    Article  CAS  Google Scholar 

  • Patra KP, Ramu T, Hoti SL, Pragasam GS, Das PK (2007) Identification of a molecular marker for genotyping human lymphatic filarial nematode parasite Wuchereria bancrofti. Exp Parasitol 116(1):59–65

    Article  CAS  Google Scholar 

  • Pradeep Kumar N, Patra KP, Hoti SL, Das PK (2002) Genetic variability of the human filarial parasite, Wuchereria bancrofti in South India. Acta Trop 82:67–76

    Article  CAS  Google Scholar 

  • Sasa M (1976) Human filariasis––a global survey of epidemiology and control. University of Tokyo Press, Tokyo, p 334

    Google Scholar 

  • Shiff C, Brouwer KC, Clow L (2000) Schistosoma haematobium: population genetics of S. haematobium by direct measurement of parasite diversity using RAPD-PCR. Exp Parasitol 96:47–51

    Article  CAS  Google Scholar 

  • Shriram AN, Sugunan AP, Murhekar MV, Sehgal SC (1996) Little Andaman Island, a new focus of infection with nocturnally periodic Wuchereria bancrofti. Indian J Med Res 104:166–170

    CAS  Google Scholar 

  • Singh J, Bhattacharji LM (1944) Rapid staining of malarial parasites by a water soluble stain. Indian Med Gaz 79:102–104

    Google Scholar 

  • Steindel M, Dias Neto E, de Menezes CL, Romanha AJ, Simpson AJ (1993) Random amplified polymorphic DNA analysis of Trypanosoma cruzi strains. Mol Biochem Parasitol 60:71–79

    Article  CAS  Google Scholar 

  • Tamura KJ, Dudley J, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24(8):1596–1599

    Article  CAS  Google Scholar 

  • Tewari SC, Hiriyan J, Reuben R (1995) Epidemiology of subperiodic Wuchereria bancrofti infection in the Nicobar Islands, India. Trans R Soc Trop Med Hyg 89(2):163–166

    Article  CAS  Google Scholar 

  • Thangadurai R, Hoti SL, Pradeep Kumar N, Das PK (2006) Phylogeography of human lymphatic filarial parasite, Wuchereria bancrofti in India. Acta Trop 98(3):297–304

    Article  CAS  Google Scholar 

  • Wilkerson RC, Parsons TJ, Albright DG, Klein TA, Braun MJ (1993) Random amplified polymorphic DNA (RAPD) markers readily distinguish cryptic mosquito species (Diptera: Culicidae: Anopheles). Insect Mol Biol 1:205–211

    Article  CAS  Google Scholar 

  • World Health Organization (2006) Global programme to eliminate lymphatic filariasis. Wkly Epidemiol Rec 81(22):221–232

    Google Scholar 

  • World Health Organization (2007) Global programme to eliminate lymphatic filariasis. Wkly Epidemiol Rec 82(42):361–380

    Google Scholar 

  • Yeh FC, Yang R (1999) PopGene v. 1.31, Microsoft Window-based freeware for population genetic analysis

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Acknowledgment

The authors thank Dr. P. K. Das, former Director, VCRC, and Dr. P. Jambulingam Director, VCRC, for their support in completing this study. One of the authors R.D is a recipient of Junior Research Fellowship from Council of Scientific and Industrial Research (CSIR, New Delhi). Also, thanks are due to Dr Francis, University of Alberta, for providing the freeware, ‘PopGene 32’, used for the analysis of data in the present study.

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Authors and Affiliations

  1. National Institute of Malaria Research (ICMR), Ranchi, India

    M. K. Das

  2. Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Pondicherry, 605 006, India

    R. Dhamodharan & S. L. Hoti

  3. National Institute of Malaria Research (ICMR), Delhi, India

    A. P. Dash

Authors
  1. M. K. Das
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  2. R. Dhamodharan
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  3. S. L. Hoti
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  4. A. P. Dash
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Correspondence to S. L. Hoti.

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Das, M.K., Dhamodharan, R., Hoti, S.L. et al. Molecular differentiation of nocturnally periodic and diurnally sub-periodic Wuchereria bancrofti by Randomly Amplified Polymorphic DNA (RAPD). World J Microbiol Biotechnol 27, 1525–1530 (2011). https://doi.org/10.1007/s11274-010-0601-6

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  • DOI: https://doi.org/10.1007/s11274-010-0601-6

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