Skip to main content
SpringerLink
Log in

Cloning and expression analysis of three secreted protein genes from wheat stripe rust fungus Puccinia striiformis f. sp. tritici

  • Short Communication
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The Puccinia striiformis f. sp. tritici (Pst) fungus is a biotrophic obligate parasite causing stripe rust, a major disease of wheat worldwide. Pst genes that encode secreted proteins serving as effectors and avirulence genes are thought to be expressed in haustoria. Three putative secreted protein genes from Pst haustoria were characterized in this study. Their full-length cDNAs were obtained using the 5′ rapid amplification of cDNA ends (RACE). Their transcripts were ranged from 769 to 1,094 base pairs (bp), encoding predicted proteins of 93 to 203 amino acids without significant similarities to any accessions in the GenBank protein database, but with some homologies to predicted proteins in P. graminis, the stem rust pathogen. The predicted products of the genes were characterized as secreted proteins and designated as PstSP2C7, PstSP11L10 and PstSP11P10. PstSP11L10 had a much higher expression level in infected leaves than in urediniospores and germinated urediniospores and therefore should be a good candidate for a gene involved in the wheat-Pst interaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Blobel G (2000) Protein targeting (Nobel lecture). Chembiochem 1:86–102

    Article  CAS  Google Scholar 

  • Catanzariti AM, Dodds PN, Lawrence GJ, Ayliffe MA, Ellis JG (2006) Haustorially expressed secreted proteins from flax rust are highly enriched for avirulence elicitors. Plant Cell 18:243–256

    Article  CAS  Google Scholar 

  • Chen XM (2005) Epidemiology and control of stripe rust on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Chen XM, Line RF (1992) Identification of stripe rust resistance genes in wheat genotypes used to differentiate North American races of Puccinia striiformis. Phytopathology 82:1428–1434

    Article  Google Scholar 

  • Dodds PN, Lawrence GJ, Catanzariti AM, Ayliffe MA, Ellis JG (2004) The Melampsora lini AvrL567 avirulence genes are expressed in haustoria and their products are recognized inside plant cells. Plant Cell 16:755–768

    Article  CAS  Google Scholar 

  • Ellis J, Dodds P, Lawrence G (2007) Flax rust resistance gene specificity is based on direct resistance-avirulence protein interactions. Annu Rev Phytopathol 45:289–306

    Article  CAS  Google Scholar 

  • Heath MC (1997) Signalling between pathogenic rust fungi and resistant or susceptible host plants. Ann Bot 80:713–720

    Article  CAS  Google Scholar 

  • Jakupović M, Heintz M, Reichmann P, Mendgen K, Hahn M (2006) Microarray analysis of expressed sequence tags from haustoria of the rust fungus Uromyces fabae. Fungal Genet Biol 43:8–19

    Article  Google Scholar 

  • Kämper J, Kahmann R, Bölker M et al (2006) Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature 444:97–101

    Article  Google Scholar 

  • Ling P, Wang MN, Chen XM, Campbell KG (2007) Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici). BMC Genomics 8:145

    Article  Google Scholar 

  • Sohn J, Voegele RT, Mendgen K, Hahn M (2000) High level activation of vitamin B1 biosynthesis genes in haustoria of the rust fungus Uromyces fabae. Mol Plant-Microbe Interact 3:629–636

    Article  Google Scholar 

  • Voegele RT, Mendgen K (2003) Rust haustoria: nutrient uptake and beyond. New Phytol 159:93–100

    Article  CAS  Google Scholar 

  • Voegele RT, Struck C, Hahn M, Mendgen K (2001) The role of haustoria in sugar supply during infection of broad bean by the rust fungus Uromyces fabae. Proc Natl Acad Sci USA 98:8133–8138

    Article  CAS  Google Scholar 

  • Voegele RT, Hahn M, Lohaus G, Link T, Heiser I, Mendgen K (2005) Possible roles for mannitol and mannitol dehydrogenase in the biotrophic plant pathogen Uromyces fabae. Plant Physiol 137:190–198

    Article  CAS  Google Scholar 

  • Yin CT, Chen XM, Wang XJ, Han QM, Kang ZS, Hulbert SH (2009) Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. tritici. BMC Genomics 10:626

    Article  Google Scholar 

  • Zhang YH, Qu ZP, Zheng WM et al (2008) Stage-specific gene expression during urediniospore germination in Puccinia striiformis f. sp tritici. BMC Genomics 9:203–212

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported in part by the Hangye Program from the Ministry of Agriculture of China (200903035-02), National Natural Science Foundation of China (No. 30930064) and the 111 Project from the Ministry of Education of China (B07049).

Author information

Authors and Affiliations

  1. College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Yan-Ling Dong & Zhen-Sheng Kang

  2. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    Yan-Ling Dong, Chun-Tao Yin, Scot Hulbert & Xian-Ming Chen

  3. USDA-ARS, Wheat Genetics, Quality, Physiology, and Disease Research Unit, Pullman, WA, 99164-6430, USA

    Xian-Ming Chen

Authors
  1. Yan-Ling Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chun-Tao Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Scot Hulbert
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xian-Ming Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhen-Sheng Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhen-Sheng Kang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dong, YL., Yin, CT., Hulbert, S. et al. Cloning and expression analysis of three secreted protein genes from wheat stripe rust fungus Puccinia striiformis f. sp. tritici . World J Microbiol Biotechnol 27, 1261–1265 (2011). https://doi.org/10.1007/s11274-010-0565-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-010-0565-6

Keywords

Search

Navigation