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Selective isolation, evaluation and characterization of antagonistic actinomycetes against Rhizoctonia solani

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Abstract

The baiting bag method was found to be useful for isolating antagonistic actinomycetes from terrestrial habitat. Out of total 110 actinomycetes isolated from rhizospheric and non-rhizospheric soil of Indo Gangetic Plains (IGP) of India, 9 isolates exhibited aggressive antagonism against Rhizoctonia solani, screened through dual culture, well diffusion and sealed plate technique. Maximum growth inhibition was recorded up to 50% under well diffusion (S. toxytricini vh22) and 52.6% in a direct confrontation (Actinomycetales bacterium vh41). Whereas maximum disease suppression (53.33%) under green house condition was achieved on seedling treated with S. tricolor vh85. Scanning electron microscopy of antagonists and pathogen interaction exhibited pore formation and hyphal degradation of test pathogen. Physiological and molecular characterization of selected isolates showed wide diversity and uncommon species has been encountered through the selective isolation technique.

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References

  • Alvindia DC, Natsuaki KT (2008) Evaluation of fungal epiphytes isolated from banana fruit surface for biocontrol of banana crown rot disease. Crop Prot 27:1200–1207

    Article  Google Scholar 

  • Berdy J (2005) Bioactive microbial metabolites. J Antibiot 58:1–26

    Article  CAS  Google Scholar 

  • Bressan W (2003) Biological control of maize seed pathogenic fungi by use of actinomycetes. Biocontrol 48:233–240

    Article  Google Scholar 

  • Cho SH, Hwang CW, Chung HK, Yang CS (1994) A new medium for the selective isolation of soil actinomycetes. J Microbiol Biotechnol 22:561–563

    Google Scholar 

  • Edwards U, Rogall TH, Blocker M, Emde BEC (1989) Isolation and direct complete nucleotide determination of entire genes, characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:843–853

    Article  Google Scholar 

  • El-Tarabily KA, Sivasithamparam K (2006) Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biol and Bioch 38:1505–1520

    Article  CAS  Google Scholar 

  • Goodfellow M, Williams E (1986) New strategies for the selective isolation of industrially important bacteria. Biotech and Genet Eng Rev 4:213–262

    CAS  Google Scholar 

  • Goodfellow M, Williams ST, Mordarski M (1988) Actinomycetes in biotechnology. Academic Press Inc, London, pp 1–88

    Google Scholar 

  • Hayakawa M, Nonomura H (1987) Humic acid vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Tech 65:501–509

    Article  CAS  Google Scholar 

  • Hermosa MR, Grondona I, Iturriaga EA, Diaz-Minguez JM, Castro C, Monte E et al (2000) Molecular characterization and identification of biocontrol isolates of Trichoderma spp. Appl Environ Microbiol 66(5):1890–1898

    Article  CAS  Google Scholar 

  • Hopwood DA, Chater K (1990) Antibiotic biosynthesis in Streptomyces (Genetics of bacterial diversity). Academic Press, London, pp 129–148

    Google Scholar 

  • Houssam MA (2009) An antifungal agent produced by Streptomyces olivaceiscleroticus AZ-SH514. World App Sci J 6:1495–1505

    Google Scholar 

  • Hsu SC, Lockwood JL (1975) Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. App Microbiol 29:422–426

    CAS  Google Scholar 

  • Kim CJ, Lee KH, Shimazu A, Kwon OS, Park DJ (1995) Isolation of rare actinomycetes on various types of soil. K J Appl Microbiol Biotechnol 23:36–42

    Google Scholar 

  • Lanoot B, Vancanneyt M, Schoor AV, Liu Z, Swings J (2005) Reclassification of Streptomyces nigrifaciens as a later synonym of Streptomyces flavovirens; Streptomyces citreofluorescens, Streptomyces chrysomallus subsp. chrysomallus and Streptomyces fluorescens as later synonyms of Streptomyces anulatus; Streptomyces chibaensis as a later synonym of Streptomyces corchorusii; Streptomyces flaviscleroticus as a later synonym of Streptomyces minutiscleroticus; and Streptomyces lipmanii, Streptomyces griseus subsp. alpha, Streptomyces griseus subsp. cretosus and Streptomyces willmorei as later synonyms of Streptomyces microflavus. Int J Syst and Evol Microbiol 55:729–731

    Article  Google Scholar 

  • Lazzarini A, Cavaletti L, Toppo G, Marinelli F (2000) Rare genera of actinomycetes as potential producers of new antibiotics. Antonie Van Leeuwenhoek 78:399–405

    Article  CAS  Google Scholar 

  • Leveau JHJ, Preston GM (2008) Bacterial mycophagy: definition and diagnosis of a unique bacterial-fungal interaction. New Phytol 177:859–876

    Article  Google Scholar 

  • Long PF, Wildman HG (1992) Strategy for the use of pretreatments in the isolation of non-streptomycete actinomycetes from soil. Actinomycetes 4:59–64

    Google Scholar 

  • Masayuki H (2008) Studies on the isolation and distribution of rare actinomycetes in soil. Actinomycetologica 22:12–19

    Article  Google Scholar 

  • Matsukawa E, Nakagawa Y, Iimura Y, Hayakawa M (2007) A new enrichment method for the selective isolation of streptomycetes from the root surfaces of herbaceous plants. Actinomycetologica 21:66–69

    Article  Google Scholar 

  • Mazzola M (2007) Manipulation of rhizosphere bacterial communities to induce suppressive soils. J Nematol 39(3):213–220

    Google Scholar 

  • Moncheva P, Tishkov S, Dimitrova N, Chipeva V, Nikolova SA, Bogatzevska N (2002) Characteristics of soil actinomycetes from Antarctica. J Cult Collect 3:3–14

    Google Scholar 

  • Nourozian J, Etebarian HR, Khodakaramian G (2006) Biological control of Fusarium graminearum on wheat by antagonistic bacteria Songklanakarin. J Sci Technol 28:29–38

    Google Scholar 

  • Pospiech A, Neumann B (1995) A versatile quick-prep of genomic DNA from gram positive bacteria. Trends Genet 11:217–218

    Article  CAS  Google Scholar 

  • Promnuan Y, Kudo T, Chantawannakul P (2009) Actinomycetes isolated from beehives in Thailand. World J Microbiol Biotechnol 25:1685–1689

    Article  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces sp. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Singh M, Sandhu RS, Randhawa HS (1987) Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum. J Clin Microbiol 24:176–177

    CAS  Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. App Microbiol 28:226–231

    CAS  Google Scholar 

  • Subramani R, Narayanasamy M (2009) Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbiol Biotech 25:2103–2211

    Article  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Bio Evo 24:1596–1599

    Article  CAS  Google Scholar 

  • Thomas GP (1965) Color and streptomycetes report of an international workshop on determination of color of streptomycetes. App Microbiol 13:43–61

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  • Tsay TT, Chen PC, Wu WS (2006) A new method for isolating and selecting agents with high antagonistic ability against plant parasitic nematodes. Plant Pathol Bull 15:9–16

    Google Scholar 

  • Walter MY, Crawford DL (1995) Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. App Environ Microbiol 61:3119–3128

    Google Scholar 

  • Williams ST, Sharpe ME, Holt JG, Murray GE, Brener DJ, Krieg NR, Mouldar JW, Pfennig NP, Sneath HA, Staley JT (1989) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore

    Google Scholar 

Download references

Acknowledgments

This study has been carried out under the network project “Application of Microorganisms in Agricultural and Allied Sectors” and financially supported by Indian Council of Agricultural Research, New Delhi, India.

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Authors and Affiliations

  1. National Bureau of Agriculturally Important Microorganisms, Mau, UP, 275101, India

    Hemant J. Patil, Alok K. Srivastava, Sudheer Kumar & Dilip K. Arora

  2. Department of Microbiology, School of Life Sciences, North Maharashtra University, Jalgaon, 425 001, India

    Bhushan L. Chaudhari

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  1. Hemant J. Patil
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  2. Alok K. Srivastava
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  3. Sudheer Kumar
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  4. Bhushan L. Chaudhari
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Correspondence to Dilip K. Arora.

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Patil, H.J., Srivastava, A.K., Kumar, S. et al. Selective isolation, evaluation and characterization of antagonistic actinomycetes against Rhizoctonia solani . World J Microbiol Biotechnol 26, 2163–2170 (2010). https://doi.org/10.1007/s11274-010-0400-0

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