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Biochemical characteristics and genetic diversity of Vibrio spp. and Aeromonas hydrophila strains isolated from the Lac of Bizerte (Tunisia)

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Abstract

This study characterized the phenotypic and genetic properties of Vibrio spp. and Aeromonas hydrophila strains isolated from seawater and mussels (Mytilus edulis and Crassostrea gigas) cultured in mollusc farm localized in the lac of Bizerte. The 37 strains (31 strains of V. alginolyticus, one strain of V. fluvialis, one strain of V. parahaemolyticus and four strains of A. hydrophila) typed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) showed a high polymorphism. Most of the isolates were resistant to at least two antimicrobial agents. All the tested strains were resistant to ampicillin. PCR was used to detect the presence of eight Vibrio cholerae virulence genes in the genome of the Vibrio spp. isolates. The results showed a wide dissemination of these genes in the genome of all Vibrio spp. isolates tested. Differentiation of these strains with the ERIC 2-PCR technique revealed no association between the presence of virulence genes and a particular fingerprinting pattern.

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Authors and Affiliations

  1. Laboratoire de Traitement des Eaux Usées. Centre de Recherches et des Technologies des eaux, Technopôle de Borj-Cédria, BP 901, 2050, Hammam-Lif, Tunisie

    Snoussi Mejdi

  2. Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Département de Microbiologie, Faculté de Pharmacie, Rue Avicenne, 5000, Monastir, Tunisie

    Snoussi Mejdi, Noumi Emira, Messaoud Ali, Hajlaoui Hafedh & Bakhrouf Amina

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  1. Snoussi Mejdi
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  2. Noumi Emira
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  3. Messaoud Ali
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  4. Hajlaoui Hafedh
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  5. Bakhrouf Amina
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Correspondence to Snoussi Mejdi.

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Snoussi Mejdi and Noumi Emira are contribute equally to the work.

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Mejdi, S., Emira, N., Ali, M. et al. Biochemical characteristics and genetic diversity of Vibrio spp. and Aeromonas hydrophila strains isolated from the Lac of Bizerte (Tunisia). World J Microbiol Biotechnol 26, 2037–2046 (2010). https://doi.org/10.1007/s11274-010-0388-5

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  • DOI: https://doi.org/10.1007/s11274-010-0388-5

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