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Characterization of tetracycline resistance genes in tetracycline-resistant Enterobacteriaceae obtained from a coliform collection

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Abstract

Tetracycline resistance has been used as the key determinant to monitor resistance genes in natural environments such as rivers, lakes or seawater. The aim of this study was evaluate the frequency of tetA, tetB, tetC, tetD and tetE genes in 52 tetracycline-resistant Enterobacteriacea isolated from river water in the North East Black Sea Region of Turkey. In 52 tetracycline-resistant strains, resistance was mediated by tetA in eight (15.3%) strains, tetB in ten (19.2%) strains and both tetA and tetB in one (1.9%) strain. No tetC, tetD or tetE-mediated resistance was detected. In conclusion, the river water may be considered as a reservoir for the antibiotic resistance genes and the people living in this area may be under risk. To our knowledge, this is the first report for molecular characterization of tetracycline resistance in Enterobacteriaceae of river water origin in Turkey.

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References

  • Aminov RI, Garrigues-Jeanjean N, Mackie RI (2001) Molecular ecology of tetracycline resistance: development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins. Appl Environ Microbiol 67:22–32

    Article  CAS  Google Scholar 

  • Aoki T, Satoh T, Kitao T (1987) New tetracycline resistance determinant on R plasmids from Vibrio anguillarum. Antimicrob Agents Chemother 31:1446–1449

    CAS  Google Scholar 

  • Bryan A, Shapir N, Sadowsky MJ (2004) Frequency and distribution of tetracycline resistance genes in genetically diverse, nonselected, and nonclinical Escherichia coli strains isolated from diverse human and animal sources. Appl Environ Microbiol 70:2503–2507

    Article  CAS  Google Scholar 

  • Chopra I, Roberts M (2001) Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev 65:232–260

    Article  CAS  Google Scholar 

  • Clinical and Laboratory Standards Institute-CLSI (2003) Performance standards for antimicrobial disk susceptibility tests; 8th ed, approved standard M2-A8., CLSI, Wayne, PA, USA

  • Col NF, O’Connor RW (1987) Estimating worldwide current antibiotic usage: report of task force I. Rev Infect Dis 9:S232–S243

    Google Scholar 

  • Dang H, Zhang X, Song L, Chang Y, Yang G (2007) Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China. J Appl Microbiol 103:2580–2592

    Article  CAS  Google Scholar 

  • DePaola A, Flynn PF, McPhearson RM, Levy SB (1988) Phenotypic and genotypic characterization of tetracycline- and oxytetracycline-resistant Aeromonas hydrophila from cultured channel catfish (Ictalurus punctatus) and their environments. Appl Environ Microbiol 54:1861–1863

    CAS  Google Scholar 

  • Dominguez E, Zarazaga M, Saenz Y, Briñas L, Torres C (2002) Mechanisms of antibiotic resistance in Escherichia coli isolates obtained from healthy children in Spain. Microb Drug Resist 8:321–327

    Article  CAS  Google Scholar 

  • Frazzon APG, Gama BA, Hermes V, Bierhals CG, Pereira RI, Guedes AG, d’Azevedo PA, Frazzon J (2010) Prevalence of antimicrobial resistance and molecular characterization of tetracycline resistance mediated by tet(M) and tet(L) genes in Enterococcus spp. isolated from food in Southern Brazil. World J Microbiol Biotechnol 26:365–370

    Article  CAS  Google Scholar 

  • Guardabassi L, Dijkshoorn L, Collard JM, Olsen JE, Dalsgaard A (2000) Distribution and in vitro transfer of tetracycline resistance determinants in clinical and aquatic Acinetobacter strains. J Med Microbiol 49:929–936

    CAS  Google Scholar 

  • Guerra B, Junker E, Schroeter A, Malorny B, Lehmann S, Helmuth R (2003) Phenotypic and genotypic characterization of antimicrobial resistance in German Escherichia coli isolates from cattle, swine and poultry. J Antimicrob Chemother 52:489–492

    Article  CAS  Google Scholar 

  • Jones JG, Gardener S, Simon BM, Pickup RW (1986) Antibiotic resistant bacteria in Windermere and two remote upland tarns in the English Lake district. J Appl Bacteriol 60:443–453

    CAS  Google Scholar 

  • Karami N, Nowrouzian F, Adlerberth I, Wold AE (2006) Tetracycline resistance in Escherichia coli and persistence in the infantile colonic microbiota. Antimicrob Agents Chemother 50:156–161

    Article  CAS  Google Scholar 

  • Lanz R, Kuhnert P, Boerlin P (2003) Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland. Vet Microbiol 91:73–84

    Article  CAS  Google Scholar 

  • Leverstein-van Hall MA, Box ATA, Blok HEM, Paauw A, Fluit AC, Verhoef J (2001) Evidence of extensive interspecies transfer of integron-mediated antimicrobial resistance genes among multidrug-resistant Enterobacteriaceae in a clinical setting. J Infect Dis 186:49–56

    Article  Google Scholar 

  • Marshall B, Tachibana C, Levy SB (1983) Frequency of tetracycline resistance determinant classes among lactose fermenting coliforms. Antimicrob Agents Chemother 24:835–840

    CAS  Google Scholar 

  • Nawaz M, Khan AA, Khan S, Sung K, Kerdahi K, Steele R (2009) Molecular characterization of tetracycline-resistant genes and integrons from avirulent strains of Escherichia coli isolated from catfish. Foodborne Pathog Dis 6:553–559

    Article  CAS  Google Scholar 

  • Ozgumus OB, Celik-Sevim E, Alpay-Karaoglu S, Sandalli C, Sevim E (2007) Molecular characterization of antibiotic resistant Escherichia coli strains isolated from tap and spring waters in a coastal region in Turkey. J Microbiol 45:379–387

    CAS  Google Scholar 

  • Ozgumus OB, Sandalli C, Sevim A, Sevim EC, Sivri N (2009) Class 1 and class 2 integrons and plasmid-mediated antibiotic resistance in coliforms isolated from ten rivers in northern Turkey. J Microbiol 47:19–27

    Article  CAS  Google Scholar 

  • Schmidt AS, Bruun MS, Dalsgaard I, Larsen JL (2001) Incidence, distribution, and spread of tetracycline resistance determinants and integron-associated antibiotic resistance genes among motile aeromonads from a fish farming environment. Appl Environ Microbiol 67:5675–5682

    Article  CAS  Google Scholar 

  • Taylor DE, Chau A (1996) Tetracycline resistance mediated by ribosomal protection. Antimicrob Agents Chemother 40:1–5

    CAS  Google Scholar 

Download references

Acknowledgments

This research was funded by the Scientific and Technological Research Council of Turkey–TUBITAK (Project No. 105T315).

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Authors and Affiliations

  1. Microbiology & Molecular Biology Research Laboratory, Department of Biology, Faculty of Arts & Sciences, Rize University, 53100, Rize, Turkey

    Cemal Sandalli, Osman Birol Özgümüş & Ali Sevim

  2. Department of Medical Microbiology, Faculty of Medicine, Rize University, 53100, Rize, Turkey

    Osman Birol Özgümüş

Authors
  1. Cemal Sandalli
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  2. Osman Birol Özgümüş
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  3. Ali Sevim
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Correspondence to Cemal Sandalli.

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Sandalli, C., Özgümüş, O.B. & Sevim, A. Characterization of tetracycline resistance genes in tetracycline-resistant Enterobacteriaceae obtained from a coliform collection. World J Microbiol Biotechnol 26, 2099–2103 (2010). https://doi.org/10.1007/s11274-010-0381-z

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  • DOI: https://doi.org/10.1007/s11274-010-0381-z

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