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Genetic diversity and plant-growth related features of Burkholderia spp. from sugarcane roots

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Abstract

Brazil is the largest sugarcane producer in the world, mainly due to the development of different management strategies. Recently, microbial-plant related studies revealed that bacterial isolates belonging to the genus Burkholderia are mainly associated with this plant and are responsible for a range of physiological activity. In this study, we properly evaluate the physiological activity and genetic diversity of endophytic and rhizospheric Burkholderia spp. isolates from sugarcane roots grown in the field in Brazil. In total, 39 isolates previously identified as Burkholderia spp. were firstly evaluated for the capability to fix nitrogen, produce siderophores, solubilise inorganic phosphates, produce indole-acetic acid and inhibit sugarcane phytopathogens in vitro. These results revealed that all isolates present at least two positive evaluated activities. Furthermore, a phylogenetic study was carried out using 16S rRNA and gyrB genes revealing that most of the isolates were affiliated with the Burkholderia cepacia complex. Hence, a clear separation given by endophytic or rhizospheric niche occupation was not observed. These results presented an overview about Burkholderia spp. isolates from sugarcane roots and supply information about the physiological activity and genetic diversity of this genus, given direction for further studies related to achieve more sustainable cultivation of sugarcane.

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Acknowledgments

This work was supported by FAPESP (Foundation for Research Assistance of São Paulo State, Brazil) (Proc. 08/52407-9) and CNPq (National Council of Research, Brazil) awarded the fellowship to D. M. Luvizotto. We also thank Armando C. F. Dias and Maria C. Quecine and José A. Silva for technical supplying and critical discussions.

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Authors and Affiliations

  1. Graduate Program in Agricultural Microbiology, Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo, Piracicaba, SP, Brazil

    Danice M. Luvizotto

  2. Department of Genetics, Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo, Piracicaba, SP, Brazil

    Danice M. Luvizotto, Francisco Dini-Andreote & Aline A. Pizzirani-Kleiner

  3. Biotechnology Center of Amazônia, Manaus, AM, Brazil

    Joelma Marcon

  4. Department of Soil Science, Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo, Piracicaba, SP, Brazil

    Fernando D. Andreote

  5. Laboratory of Molecular Biology and Microbial Ecology, NIB, University of Mogi das Cruzes, Mogi das Cruzes, SP, 08780-911, Brazil

    Aline A. C. Neves & Welington L. Araújo

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  1. Danice M. Luvizotto
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  2. Joelma Marcon
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Correspondence to Welington L. Araújo.

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Luvizotto, D.M., Marcon, J., Andreote, F.D. et al. Genetic diversity and plant-growth related features of Burkholderia spp. from sugarcane roots. World J Microbiol Biotechnol 26, 1829–1836 (2010). https://doi.org/10.1007/s11274-010-0364-0

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  • DOI: https://doi.org/10.1007/s11274-010-0364-0

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