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Colonization of endophyte Pantoea agglomerans YS19 on host rice, with formation of multicellular symplasmata

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Abstract

Pantoea agglomerans YS19 is a diazotrophic endophyte isolated from rice (Oryza sativa cv. Yuefu) grown in a temperate-climatic region in west Beijing (China). The colonization of YS19 on host rice was studied in this paper. It was revealed that YS19 colonizes in all the tissues of rice seedlings, including roots (dominantly at elongation regions, lateral root junctions, root hairs and root caps), stems and leaves. More YS19 colonizes in stem and leaves (1.40 × 105 CFU mg−1 fresh weight) than that in roots (3.60 × 104 CFU mg−1). Symplasmata, a kind of adaptive structure of the strain for its endophytic living, were repeatedly observed to form inside root or stem cortex parenchyma tissues, as well as on leaf surfaces and also rhizoplanes. A novel matrix protein (SPM43.1) with its expression paralleling to the formation of symplasmata was captured, whose meaning in structural construction of symplasmata was also discussed.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30870055 and 30871464). The authors are grateful to Mr. Y. Miao (Beijing Institute of Technology) for his constructive suggestions.

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Authors and Affiliations

  1. School of Life Science, Beijing Institute of Technology, 5 Zhongguancun Nandajie, Haidian District, 100081, Beijing, People’s Republic of China

    Xiao Zhang, Erli Li, Xiaolu Xiong & Yongjun Feng

  2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Delong Shen

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  1. Xiao Zhang
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  2. Erli Li
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  3. Xiaolu Xiong
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  4. Delong Shen
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  5. Yongjun Feng
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Correspondence to Yongjun Feng.

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Zhang, X., Li, E., Xiong, X. et al. Colonization of endophyte Pantoea agglomerans YS19 on host rice, with formation of multicellular symplasmata. World J Microbiol Biotechnol 26, 1667–1673 (2010). https://doi.org/10.1007/s11274-010-0344-4

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