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A potential bacterial biocontrol agent, strain S2V2 against pathogenic marine Vibrio in aquaculture

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Abstract

We isolated a marine bacterium strain S2V2 which inhibited the growth of pathogenic marine Vibrio spp. The aims of this research were to identify a new antibiotic-producing marine bacterium strain S2V2, and evaluate its spectrum activity and pathogenic property. Analysis of 16S rDNA sequence placed strain S2V2 in the genus Pseudoalteromonas, but the sequence similarity was low (95.46%) implying the strain might be a new species in this genus. Strain S2V2 inhibited the growth of 67.9% of 28 Vibrio strains tested. This strain inhibited V. alginolyticus, V. anguillarum, V. fluvialis, V. harveyi, V. metschnikovii, V. splendidus, V. ordalii, V. parahaemolyticus, and V. vulnificus, but inactive against V. campbellii, Aeromonas hydrophyla and Staphylococcus aureus. Strain S2V2 produced extracellular non proteinaceous antibacterial substances. The highest antibacterial activity was found when strain S2V2 was cultured for 96 h in ZoBell broth medium. An artificial infection to post larvae of Lithopenaeus vanname indicated that strain S2V2 was a non pathogenic bacterium. Non pathogenic property and specific antibacterial activity against a broad range of fish pathogenic marine Vibrio of strain S2V2 suggest that this strain is a prospective source of unique antibiotic and a potential biocontrol agent in marine aquaculture.

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Acknowledgments

Identification of strain S2V2 was financially supported by a grant from Directorate of Higher Education, Department of National Education, the Republic of Indonesia under Fundamental Research Program (Contract number: LPPM-UGM/568/2007). We thank to the Laboratory of Fish and Environment Health, Brackishwater Aquaculture Development Center, Jepara, Indonesia for the gift of V. harveyi isolate.

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  1. Laboratory of Fish Diseases, Department of Fisheries, Faculty of Agriculture, Gadjah Mada University, Jl. Flora Bulaksumur, Yogyakarta, 55182, Indonesia

    Alim Isnansetyo, Indah Istiqomah,  Muhtadi, Shofihar Sinansari, Rudi Kurnia Hernawan &  Triyanto

  2. Laboratory of Agricultural Microbiology, Faculty of Agriculture, Gadjah Mada University, Jl. Flora, Bulaksumur, Yogyakarta, 55182, Indonesia

    Jaka Widada

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  1. Alim Isnansetyo
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Isnansetyo, A., Istiqomah, I., Muhtadi et al. A potential bacterial biocontrol agent, strain S2V2 against pathogenic marine Vibrio in aquaculture. World J Microbiol Biotechnol 25, 1103–1113 (2009). https://doi.org/10.1007/s11274-009-9992-7

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