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Bio-sequestration of carbon dioxide using carbonic anhydrase enzyme purified from Citrobacter freundii

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Abstract

The increase in the atmospheric concentrations of one of the vital green house gasses, carbon dioxide, due to anthropogenic interventions has led to several undesirable consequences such as global warming and related changes. In the global effort to combat the predicted disaster, several CO2 capture and storage technologies are being deliberated. One of the most promising biological carbon dioxide sequestration technologies is the enzyme catalyzed carbon dioxide sequestration into bicarbonates which was endeavored in this study with a purified C. freundii SW3 β-carbonic anhydrase (CA). An extensive screening process for biological sequestration using CA has been defined. Six bacteria with high CA activity were screened out of 102 colonies based on plate assay and presence of CA in these bacteria was further emphasized by activity staining and Western blot. The identity of selected bacteria was confirmed by 16S rDNA analysis. CA was purified to homogeneity from C. freundii SW3 by subsequent gel filtration and ion exchange chromatography which resulted in a 24 kDa polypeptide and this is in accordance with the Western blot results. The effect of host on metal ions, cations and anions which influence activity of the enzyme in sequestration studies suggests that mercury and HCO3 − ion almost completely inhibit the enzyme whereas sulfate ion and zinc enhances carbonic anhydrase activity. Calcium carbonate deposition was observed in calcium chloride solution saturated with carbon dioxide catalyzed by purified enzyme and whereas a sharp decrease in calcium carbonate formation has been noted in purified enzyme samples inhibited by EDTA and acetazolamide.

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Acknowledgments

Mr. R. Rishiram is a recipient of Senior Research Fellowship from University Grants Commission (UGC) and thanks the UGC for financial assistance. The authors also thank Department of Biotechnology, Government of India for the financial support provided for this project.

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Authors and Affiliations

  1. Environmental Biotechnology Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, 440020, Maharashtra, India

    Rishiram Ramanan, Krishnamurthi Kannan, Saravana Devi Sivanesan, Sandeep Mudliar & Tapan Chakrabarti

  2. School of Biotechnology, Banaras Hindu University, Varanasi, India

    Simarjot Kaur & Anil Kumar Tripathi

Authors
  1. Rishiram Ramanan
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  2. Krishnamurthi Kannan
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  3. Saravana Devi Sivanesan
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  4. Sandeep Mudliar
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  5. Simarjot Kaur
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  6. Anil Kumar Tripathi
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  7. Tapan Chakrabarti
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Correspondence to Krishnamurthi Kannan.

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Ramanan, R., Kannan, K., Sivanesan, S.D. et al. Bio-sequestration of carbon dioxide using carbonic anhydrase enzyme purified from Citrobacter freundii . World J Microbiol Biotechnol 25, 981–987 (2009). https://doi.org/10.1007/s11274-009-9975-8

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  • DOI: https://doi.org/10.1007/s11274-009-9975-8

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