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Mode of antagonism of a biocontrol bacterium Lysobacter sp. SB-K88 toward a damping-off pathogen Aphanomyces cochlioides

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Abstract

The biocontrol bacterium Lysobacter sp. SB-K88 suppresses damping-off disease in sugar beet and spinach caused by Aphanomyces cochlioides and Pythium sp. through characteristic plant colonization and antibiosis against the pathogens. This study aimed to unravel further details on mode of antagonism of SB-K88 against a damping-off pathogen A. cochlioides AC-5. The SB-K88 substantially inhibited growth and decomposed AC-5 mycelia and suppressed the release of zoospores from the hyphae. The excised root tips of sugar beet seedlings from seeds previously inoculated with SB-K88 were less attractive to AC-5 zoospores. Although aerial growth was not affected, however, root hairs of SB-K88 inoculated sugar beet seedlings were remarkably shorter and thicker than those of uninoculated control. When exposed to zoospores, the SB-K88 inhibited motility of zoospores and/or caused lysis, and then aggregated around the dead cystospores or lysed residues within 3–6 h likely to be micro-predatory behavior to a eukaryotic organism. Confocal laser scanning microscopic analysis revealed that number of lipid bodies and activities of mitochondria were markedly increased in the affected hyphae compared with control hyphae as visualized by established vital stains. Taken together, these results suggest that Lysobacter sp. SB-K88 suppresses damping-off diseases through exerting multifaceted antagonistic effects against the peronosporomycetes.

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Acknowledgments

I’m very thankful to Prof. Dr. Satoshi Tahara and Prof. Yasuyuki Hashidoko for their kind supports and encouragements during this work at Laboratory of Ecological Chemistry of Hokkaido University, Japan. My sincere thanks are also due to Prof. R. Yokosawa (Health Science University of Hokkaido, Japan) and Dr. Y. Homma and Dr. T. Nakayama (National Agricultural Research Center for Hokkaido Region, Sapporo, Japan) for their kind gifts of A. cochlioides AC-5 and Lysobacter sp. SB-K88, respectively. Dr. A. Deora, T. Ito, and Y. Aoyama, Graduate School of Agriculture of Hokkaido University, Japan deserve thanks for their assistance during SEM and CLSM studies. deserve special The financial support and a Postdoctoral Fellowship from the Japan Society for the Promotion of Science (JSPS) are also very much appreciated.

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  1. Graduate School of Agriculture, Hokkaido University, Kita-Ku, Sapporo, 060-8589, Japan

    Md. Tofazzal Islam

  2. School of Agriculture and Rural Development, Bangladesh Open University, Gazipur, 1705, Bangladesh

    Md. Tofazzal Islam

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Tofazzal Islam, M. Mode of antagonism of a biocontrol bacterium Lysobacter sp. SB-K88 toward a damping-off pathogen Aphanomyces cochlioides . World J Microbiol Biotechnol 26, 629–637 (2010). https://doi.org/10.1007/s11274-009-0216-y

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