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The potential use of bacterium strain R219 for controlling of the bloom-forming cyanobacteria in freshwater lake

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Abstract

Cyanobacterial blooms become a serious environmental threat to the freshwater ecosystem, and several physical and chemical methods have been developed for controlling the blooms. In order to develop a biocontrol agent for controlling the blooms, we isolated a bacterial strain R219 that exhibited strong algicidal activity against the dominant bloom-forming species of Microcystis aeruginosa from Lake Tai in China. Based on 16S rDNA sequence analysis we determined the strain R219 to be Pseudomonas aeruginosa by the virtue of its sharing about 99.8% similarity with reference strains in the DNA databases. Biochemical and morphological tests were used to support the accurate identification as that of the bacterium P. aeruginosa. We also tested culture filtrate and ethyl acetate extract of strain R219 and showed both of them exhibited strong algicidal effect on the growth of M. aeruginosa at mid-exponential phase when the R219 filtrate and ethyl acetate extract were applied at various cell densities. Moreover, the P. aeruginosa filtrate showed high potency in removal of the mixed species bloom-forming cyanobacteria collected directly from the Lake Tai. When adding the filtrate of the strain R219 to the mixed-species cyanobacteria, the content of chlorophyll-a of the algae were reduced by as much as 80–90%. Oral acute toxicity assessment for strain R219 demonstrated that all the mice that received the broth or filtrate in doses of 0.5 or 2.0 g kg−1 were alive without any immediate behavioral changes within 14 days of administration of either broth or filtrate. These results indicate that the strain R219 may have potential for a use in controlling the bloom-forming cyanobacteria in freshwater ecosystems.

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Acknowledgments

This work was supported by the National Basic Research Program of China (2008CB418004) and the Special Program for Taihu Lake of Jiangsu Natural Science Foundation (BK2007739), for which the authors are thankful. Special thanks for Professor Lubomir Sokolov who made a lot of valuable comments during the manuscript preparation.

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Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Jiangsu, China

    Hongqin Ren, Ping Zhang, Changhong Liu & Yarong Xue

  2. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, China

    Bin Lian

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  1. Hongqin Ren
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  2. Ping Zhang
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  3. Changhong Liu
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  4. Yarong Xue
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  5. Bin Lian
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Correspondence to Changhong Liu or Bin Lian.

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Ren, H., Zhang, P., Liu, C. et al. The potential use of bacterium strain R219 for controlling of the bloom-forming cyanobacteria in freshwater lake. World J Microbiol Biotechnol 26, 465–472 (2010). https://doi.org/10.1007/s11274-009-0192-2

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  • DOI: https://doi.org/10.1007/s11274-009-0192-2

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