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Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes

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Abstract

In this study, Bacillus sphaericus NRC 69 was grown in culture media, in which 12 agricultural wastes were tested as the main carbon, nitrogen and energy sources under solid state fermentation. Of the 12 tested agricultural by-products, wheat bran was the most efficient substrate for the production of B. sphaericus mosquitocidal toxins against larvae of Culex pipiens (LC50 1.2 ppm). Mixtures of tested agricultural wastes separately with wheat bran enhanced the produced toxicity several folds and decreased LC50 between 3.7- and 50-fold in comparison with that of agricultural wastes without mixing. The toxicity of B. sphaericus grown in wheat bran/rice hull at 8/2 (g/g) and wheat bran/barley straw at 1/4 (g/g) showed the same toxicity as that in wheat bran medium (LC50 decreased 17- and 16-fold, in comparison with that in rice hull or barely straw media, respectively). In wheat bran medium, the maximum toxicity of the tested organism obtained at 50% moisture content, inoculum size 84 × 106 CFU/g wheat bran and incubation for 6 days at 30°C. Addition of cheese whey permeate at 10% to wheat bran medium enhanced the toxicity of B. sphaericus NRC 69 about 46%.

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  1. Microbial Chemistry Department, National Research Centre, El-Behoos Street, Doki, Giza, Egypt

    Magda A. El-Bendary

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  1. Magda A. El-Bendary
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Correspondence to Magda A. El-Bendary.

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El-Bendary, M.A. Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes. World J Microbiol Biotechnol 26, 153–159 (2010). https://doi.org/10.1007/s11274-009-0154-8

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  • DOI: https://doi.org/10.1007/s11274-009-0154-8

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