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Phytochemical analysis of Andrographis paniculata extract and its antimicrobial activity

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Abstract

The present study describes the phytochemical profile and antimicrobial activity of Andrographis paniculata. For the present investigation, two samples of A. paniculata extracts, obtained by extraction in chloroform and chloroform + HCl, respectively, were compared for their antimicrobial activity and further subjected to GC-MS analysis to find out the nature of the compounds responsible for the antimicrobial activity. The antibacterial activities were assessed by measuring the diameter of the inhibition zones, MIC and MBC values. Compared to the chloroform + HCl extract, the chloroform extract showed better antimicrobial activity against all the nine pathogenic bacterial strains tested. The chloroform extract was observed to be active against the opportunistic and pathogenic gram-negative bacteria, indicating its potential application related to noscomial infections. GC-MS results revealed phenols, aromatic carboxylic acids and esters in the chloroform extract to be the molecules responsible for the antimicrobial activity of A. paniculata. This is the first report on analysis of antimicrobial components from A. paniculata, and our results confer the utility of this plant extract in developing a novel broad spectrum antimicrobial agent.

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Acknowledgments

AG acknowledges financial assistance from AICTE, New Delhi, India.

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Authors and Affiliations

  1. Biotechnology Department, Chaitanya Bharathi Institute of Technology, Hyderabad, India

    Soma Roy

  2. Center for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Andhra Pradesh, 500 085, India

    Kiranmayee Rao, Ch. Bhuvaneswari, Archana Giri & Lakshmi Narasu Mangamoori

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  1. Soma Roy
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  2. Kiranmayee Rao
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  3. Ch. Bhuvaneswari
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  4. Archana Giri
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  5. Lakshmi Narasu Mangamoori
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Correspondence to Archana Giri.

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Roy, S., Rao, K., Bhuvaneswari, C. et al. Phytochemical analysis of Andrographis paniculata extract and its antimicrobial activity. World J Microbiol Biotechnol 26, 85–91 (2010). https://doi.org/10.1007/s11274-009-0146-8

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  • DOI: https://doi.org/10.1007/s11274-009-0146-8

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