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Bioactive potential of Streptomyces associated with marine sponges

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Abstract

The present work deals with isolation of Streptomyces associated with marine sponges and its bioactive potential. Streptomyces sp. were isolated from the marine sponges Callyspongia diffusa, Mycale mytilorum, Tedania anhelans and Dysidea fragilis. From the initial screening, 94 cultures of Streptomyces were obtained and from these 58 cultures exhibited antagonism against bacteria, 36 strains against fungi and 27 strains exhibited broad spectrum activity against both. The submerged culture extracts of the 58 anti-bacterial isolates were analysed and of these 58 strains, 37 strains showed positive inhibition against Bacillus subtilis, 43 against Staphylococcus aureus, 10 against Vibrio cholerae and 10 against Escherichia coli. The antifungal activities of the 36 strains were also evaluated and 27 strains showed positive inhibition against Aspergillus niger, 23 against Saccharomyces cerevisiae and 16 against Candida albicans. The production of polyene substances from the active extracts was confirmed by UV spectral analysis by the absorbance peaks that ranged from 225 to 262 nm and the TLC (R f values) ranging from 0.40 to 0.78. The results indicate that Streptomyces strains isolated from marine sponges produce potential antibacterial, antifungal and broad spectrum antibiotic compounds.

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Acknowledgments

The authors wish to thank Dr P. A. Thomas, retired scientist, CMFRI, Vizhinjam, Kerala, India for his help in identification of sponges and DST-SERC (SR/S0/AS-57/2004) New Delhi, for financial support.

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Authors and Affiliations

  1. Department of Aquatic Biology and Fisheries, University of Kerala, Kariavattom campus, Trivandrum, 695581, India

    Selvakumar Dharmaraj

  2. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, 695019, India

    Alagarsamy Sumantha

  3. 9/36, Second Street, Park Avenue, Thudiyalur, Coimbatore, Tamil Nadu, 641034, India

    Selvakumar Dharmaraj

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  1. Selvakumar Dharmaraj
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Correspondence to Selvakumar Dharmaraj.

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Dharmaraj, S., Sumantha, A. Bioactive potential of Streptomyces associated with marine sponges. World J Microbiol Biotechnol 25, 1971–1979 (2009). https://doi.org/10.1007/s11274-009-0096-1

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