Abstract
In order to correlate cyclopropane fatty acid of the membrane of Lactobacillus bulgaricus L2 with freeze-drying survival at different growth conditions, fatty acid methyl esters (FAME) from extracts grown at difference fermentation pH (5.0, 5.5, 6.0, 6.5) and temperature (30, 35, 37, 39°C) were obtained and analyzed. Results showed that cultures grown at 30°C and pH 5.0, 35°C and pH 5.0, 39°C and pH 6.0 exhibited more resistance to the freeze-drying process than cultures grown in other conditions, cells cultured at 30°C and pH 5.0 had a highest survival rate. On the other hand, cells grown at 37°C displayed poor resistance to adverse conditions possible because of the lower cycC19:0 content. It was concluded that the improved cryotolerance observed during freeze-drying would be associated with an increase in cycC19:0 content and cycC19:0/SFA ratio and vice versa.
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Acknowledgments
This work was supported by the Key Laboratory of Dairy Science, Ministry of Education (Northeast Agricultural University). We are grateful to Mr. Axel Rau for the helpful manuscript revision. We also thank Dr. Li-Bo Liu for statistical calculations.
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Li, C., Zhao, JL., Wang, YT. et al. Synthesis of cyclopropane fatty acid and its effect on freeze-drying survival of Lactobacillus bulgaricus L2 at different growth conditions. World J Microbiol Biotechnol 25, 1659–1665 (2009). https://doi.org/10.1007/s11274-009-0060-0
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DOI: https://doi.org/10.1007/s11274-009-0060-0