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Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5

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Abstract

In a screening program for isolation of thermophilic lipase-producing bacteria, a number of thermophilic bacteria were isolated from desert soil from Baltim, Egypt. Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60°C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94–100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30–60°C. The enzyme retained 84.84% of its original activity during incubation at 70°C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield.

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Acknowledgment

M. Sifour is very grateful to the “Ministry of Higher Education and Scientific Research of Algeria” for its financial support.

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Authors and Affiliations

  1. Department of Environmental Science, Faculty of Science (Moharam Bay), University of Alexandria, Alexandria, Egypt

    Mahmoud M. Berekaa

  2. Department of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, Alexandria, Egypt

    Taha I. Zaghloul & Hesham M. Saeed

  3. Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt

    Yasser R. Abdel-Fattah

  4. Department of Molecular and Cell Biology, Faculty of Science, University of Jijel, Jijel, Algeria

    Mohamed Sifour

Authors
  1. Mahmoud M. Berekaa
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  2. Taha I. Zaghloul
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  3. Yasser R. Abdel-Fattah
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  4. Hesham M. Saeed
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  5. Mohamed Sifour
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Correspondence to Mahmoud M. Berekaa.

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Berekaa, M.M., Zaghloul, T.I., Abdel-Fattah, Y.R. et al. Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5. World J Microbiol Biotechnol 25, 287–294 (2009). https://doi.org/10.1007/s11274-008-9891-3

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  • DOI: https://doi.org/10.1007/s11274-008-9891-3

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