Abstract
A marine Streptomyces sp. 060524 capable of hydrolyzing the glycosidic bond of isoflavone glycosides, was isolated by detecting its β-glucosidase activity. 5 isoflavone aglycones were isolated from culture filtrates in soybean meal glucose medium. They were identified as genistein (1), glycitein (2), daidzein (3), 3′,4′,5,7-tetrahydroxyisoflavone (4), and 3′,4′,7-trihydroxyisoflavone (5), based on UV, NMR and mass spectral analysis. The Streptomyces can selectively hydroxylate at the 3′-position in the daidzein and genistein to generate 3′-hydroxydaidzein and 3′-hydroxygenistein, respectively. The Strain biotransformed more than 90% of soybean isoflavone glycosides into their aglycones within 108 h. 3′-hydroxydaidzein and 3′-hydroxygenistein exhibited stronger cytotoxicity against K562 human chronic leukemia than daidzein and genistein.
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This work was co-supported by a project for HK from China High-Tech Research and Development Program (863) (2002AA628140), a grant for YCS from National Natural Science Foundation of China (30300007), and the key grant (No. 104195) for RXT from The Ministry of Education.
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Hu, SC., Hong, K., Song, YC. et al. Biotransformation of soybean isoflavones by a marine Streptomyces sp. 060524 and cytotoxicity of the products. World J Microbiol Biotechnol 25, 115–121 (2009). https://doi.org/10.1007/s11274-008-9872-6
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DOI: https://doi.org/10.1007/s11274-008-9872-6