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Proteolytic breakdown of gliadin by Enterococcus faecalis isolated from Tunisian fermented dough

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Abstract

The aim of this work was to select strains with proteolytic activity on wheat gliadin, among lactic acid bacteria, previously isolated from Tunisian fermented wheat dough. Hydrolysis of gliadin, as sole nitrogen source, in an agar medium was visualized by a clear zone surrounding colonies. The increase in absorbance due to gliadin breakdown was measured spectrophotometrically using O-phthaldialdehyde (OPA) on Gliadin Glucose Broth medium. Fermented liquid dough inoculated with individual selected Enterococcus faecalis, showed a decrease of the gliadin concentration from 45 g/kg to 18 g/kg determined by sandwich ELISA test (R-7001). Only the enterococci strains show an hydrolysis of gliadin proteins. Strains showing proteolytic activity are gaining more and more importance in cereal based fermented foods and may help to reduce gliadin involved in coeliac disease.

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Acknowledgements

The authors acknowledge financial support for this work from the AUF cooperation (project AUF-6301PS338). We appreciated the bacteriological work accomplished by Dr. D. Janssens, BCCM/LMG, University of Gent, Belgium. We gratefully acknowledge Mrs. Muggy PETTRÉ and Pr. Claude DEROANNE for using Glutomatic and also Pr. Daniel PORTETELLE for reading ELISA test (Faculté Universitaire des Sciences Agronomiques de Gembloux, Belgium).

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Authors and Affiliations

  1. Laboratoire d’Écologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie (I.N.S.A.T)—Boulevard de la Terre, Centre Urbain Nord de Tunis, BP 676, 1080, Tunis, Tunisia

    Sana M’hir, Mondher Mejri & Moktar Hamdi

  2. Centre Wallon de Biologie Industrielle (C.W.B.I.)—Unité de Bio-Industries, Faculté Universitaire des Sciences Agronomiques (Fusagx)—Passage des Déportés, 2-B, 5030, Gembloux, Belgium

    Jean-Marc Aldric, Thami El-Mejdoub, Jaqueline Destain & Phillippe Thonart

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  1. Sana M’hir
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  2. Jean-Marc Aldric
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  3. Thami El-Mejdoub
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  4. Jaqueline Destain
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  5. Mondher Mejri
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  6. Moktar Hamdi
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  7. Phillippe Thonart
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Correspondence to Moktar Hamdi.

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M’hir, S., Aldric, JM., El-Mejdoub, T. et al. Proteolytic breakdown of gliadin by Enterococcus faecalis isolated from Tunisian fermented dough. World J Microbiol Biotechnol 24, 2775–2781 (2008). https://doi.org/10.1007/s11274-008-9804-5

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  • DOI: https://doi.org/10.1007/s11274-008-9804-5

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