Abstract
A strain utilizing cyclohexylamine as the sole source of carbon and nitrogen, designated NyZ12, was isolated from soil and identified by 16S rDNA sequencing as Pseudomonas plecoglossicida. This bacterium released ammonia into the medium when grown on cyclohexylamine, and also grows readily on cyclohexanone as the sole carbon source, suggesting that degradation involves an initial deamination step.
References
Bopp BA, Sonders RC, Kesterson JW (1986) Toxicological aspects of cyclamate and cyclohexylamine. Crit Rev Toxicol 16:213–306
Ferre E, N`guyen MH, Le Petit J et al (2002) Microbial oxidation of amines: a preliminary investigation on the resolution of racemic 1-phenylethyl amine. Biocatal Biotransform 20:311–317
Hwang DF, Chang SH, Shiua CY et al (1997) High performance liquid chromato- graphic determination of biogenic amines in fish implicated in food poisoning. J Chromatogr B 693:23–30
Iwaki H, Shimizu M, Tokuyama T et al (1999) Biodegradation of cyclohexylamine by Brevibacterium oxydans IH-35A. Appl Environ Microbiol 65:2232–2234
Kroes R, Peters PW, Berkvens JM et al (1977) Long term toxicity and reproduction study (including a teratogenicity study) with cyclamate, saccharin and cyclohexylamine. Toxicology 8:285–300
Prosky L, Odell RG (1971) In-vivo conversion of 14C-labeled cyclamate to cyclohexylamine. J Pharm Sci 60:1341–1343
Renwick AG, Williams RT (1972) The metabolites of cyclohexylamine in man and certain animals. Biochem J 129:869–879
Shi YZ, Xiong XX, Chen XX (1998) The chromogenic reaction of ammonium ion with Nessler’s reagent and the nitrogen determination by the photometric analysis. J Wuhan Yejin Univ Sci and Technol 21:40–43
Tokieda T, Niimura T, Takamura F, Yamaha T(1977) Purification and some properties of cyclohexylamine oxidase from a Pseudomonas sp. J Biochem 81:851–858
Yen GC, Hsien CL (1991) Simultaneous analysis of biogenic amines in canned fish by HPLC. J Food Sci 56:158–160
Acknowledgments
This work was supported by “Wuhan Shi Xue Ke Dai Tou Ren Ji Hua” of the Bureau of Science and Technology of Wuhan Municipality (Grant no. 20065006131-04) and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant no. KSCX2-YW-G-009).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Shen, Y., Yan, DZ., Chi, XQ. et al. Degradation of cyclohexylamine by a new isolate of Pseudomonas plecoglossicida . World J Microbiol Biotechnol 24, 1623–1625 (2008). https://doi.org/10.1007/s11274-007-9651-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-007-9651-9