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Isolation and identification of an endophytic strain of Fusarium oxysporum producing podophyllotoxin from Juniperus recurva

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Abstract

Podophyllotoxin, a well-known naturally occurring aryltetralin lignan occurs in few plant species that is used as a precursor for the chemical synthesis of the anticancer drugs like etoposide, teniposide and etopophose phosphate. The availiability of this lignan is becoming increasingly limited because of the scarce occurance of its natural sources and also because synthetic approaches for its production are still commercially unacceptable. This paper reports first time the production of podophyllotoxin by an endophytic fungus Fusarium oxysporum isolated from the medicinal plant Juniperus recurva. Further confirmation and quantification of podophyllotoxin was performed by HPLC, LC-MS, and LC-MS/MS.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Integrative Medicine (IIIM), Sanat Nagar, Srinagar, J&K, 190005, India

    Amardeep Kour, Abdul S. Shawl, Suriya Rehman, Phalisteen Sultan & Parvaiz H. Qazi

  2. Indian Institute of Integrative Medicine (IIIM), Canal Road, Jammu Tawi, J&K, India

    Pankaj Suden, Ravi K. Khajuria & Vijeshwar Verma

Authors
  1. Amardeep Kour
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  2. Abdul S. Shawl
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  3. Suriya Rehman
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  4. Phalisteen Sultan
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  5. Parvaiz H. Qazi
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  6. Pankaj Suden
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  7. Ravi K. Khajuria
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  8. Vijeshwar Verma
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Correspondence to Parvaiz H. Qazi.

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Kour, A., Shawl, A.S., Rehman, S. et al. Isolation and identification of an endophytic strain of Fusarium oxysporum producing podophyllotoxin from Juniperus recurva . World J Microbiol Biotechnol 24, 1115–1121 (2008). https://doi.org/10.1007/s11274-007-9582-5

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  • DOI: https://doi.org/10.1007/s11274-007-9582-5

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