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Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze

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Abstract

Rosmarinic acid, an important phenolic active compound, is one of the main active constituents of Agastache rugosa Kuntze and has astringent properties, antioxidant capacity, anti-inflammatory activity, antimutagenic ability, antimicrobial capacity, and antiviral properties. To investigate in vitro production of rosmarinic acid, we established a hairy root culture of A. rugosa by infecting leaf and stem explants with Agrobacterium rhizogenes R1000, and tested the growth and rosmarinic acid production of these cultures. Hairy roots were cultured in Murashige and Skoog liquid medium and maximum growth (14.1 g dry wt/l) was attained after 14 days of culture, at which time the content of rosmarinic acid was 116 mg/g dry wt. The present results demonstrate that hairy root culture of A. rugosa is a valuable alternative approach for the production of rosmarinic acid.

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References

  • Chen H, Chena F, Chiu FC, Lo CM (2001) The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza. Enzyme Microb Technol 28:100–105

    Article  CAS  Google Scholar 

  • Giri A, Narasu MJ (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18:1–22

    Article  CAS  Google Scholar 

  • Grzegorczyk I, Krolicka A, Wysokinska H (2006) Establishment of Salvia officinalis L. hairy root cultures for the production of rosmarinic acid. Z Naturforsch [C] 61:351–356

    CAS  Google Scholar 

  • Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006a) Harnessing the potential of hairy roots: dawn of a new era. Trends Biotechnol 24:403–409

    Article  CAS  Google Scholar 

  • Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006b) Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol 9:341–346

    Article  CAS  Google Scholar 

  • Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987) New routes to plant secondary products. Biotechnology 5:800–804

    Article  CAS  Google Scholar 

  • Hong JH, Choi JH, Oh SR, Lee HK, Park JH, Lee KY, Kim JJ, Jeong TS, Oh GT (2001) Inhibition of cytokine-induced vascular cell adhesion molecule-1 expression; possible mechanism for anti-atherogenic effect of Agastache rugosa. FEBS Lett 495:142–147

    Article  CAS  Google Scholar 

  • Kim HK, Oh SR, Lee HK, Huh H (2001a) Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze. Biotechnol Lett 23:55–60

    Article  Google Scholar 

  • Kim TH, Shin JH, Baek HH, Lee HJ (2001b) Volatile flavour compounds in suspension culture of Agastache rugosa Kuntze (Korean mint). J Sci Food Agric 81:569–575

    Article  CAS  Google Scholar 

  • Li W, Koike K, Asada Y, Yoshikawa T, Nikaido T (2005) Rosmarinic acid production by Coleus forskohlii hairy root cultures. Plant Cell Tissue Organ Cult 80:151–155

    Article  CAS  Google Scholar 

  • Ly TN, Shimoyamada M, Yamauchi R (2006) Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth leaves and their antioxidative activity. J Agric Food Chem 54:3786–3793

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Oh HM, Kang YJ, Kim SH, Lee YS, Park MK, Heo JM, Sun J, Kim HJ, Kang ES, Kim HJ, Seo HG, Lee JH, Yun-Choi HS, Chang KC (2005) Agastache rugosa leaf extract inhibits the iNOS expression in ROS 17/2.8 cells activated with TNF-alpha and IL-1beta. Arch Pharm Res 28:305–310

    Article  CAS  Google Scholar 

  • Parnham MJ, Kesselring K (1985) Rosmarinic acid. Drugs Future 10:756–757

    Google Scholar 

  • Petersen M, Simmonds MSJ (2003) Rosmarinic acid. Phytochemistry 62:121–125

    Article  CAS  Google Scholar 

  • Shin S, Kang CA (2003) Antifungal activity of the essential oil of Agastache rugosa Kuntze and its synergism with ketoconazole. Lett Appl Microbiol 36:111–115

    Article  CAS  Google Scholar 

  • Signs M., Flores H (1990) The biosynthetic potential of plant roots. Bioessays 12:7–13

    Article  CAS  Google Scholar 

  • Tada H, Murakami Y, Omoto T, Shimomura K, Ishimaru K (1996) Rosmarinic acid and related phenolics in hairy root cultures of Ocimum basilicum. Phytochemistry 42:431–434

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by research funds from Chosun University, 2007.

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Authors and Affiliations

  1. Research Center for Proteineous Materials, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Korea

    Sook Young Lee

  2. Division of Plant Science and Resources, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-754, Korea

    Hui Xu, Yong Kyung Kim & Sang Un Park

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  1. Sook Young Lee
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  2. Hui Xu
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  4. Sang Un Park
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Correspondence to Sang Un Park.

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Lee, S.Y., Xu, H., Kim, Y.K. et al. Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze. World J Microbiol Biotechnol 24, 969–972 (2008). https://doi.org/10.1007/s11274-007-9560-y

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  • DOI: https://doi.org/10.1007/s11274-007-9560-y

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