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Nitrite utilization by Chaetoceros muelleri under elevated CO2 concentration

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Abstract

Chaetoceros muelleri (Lemn.) was cultured with nitrite (NO −2 ) or nitrate (NO −3 ) as the sole nitrogen source and aerated with air or with CO2-enriched air. Cells of C. muelleri excreted into the medium nitrite produced by reduction of nitrate when grown with 100 μM NaNO3 as nitrogen source. Accordingly, NO −2 concentration reached 10.4 μM after 95 h at the low CO2 condition (aerated with air); while the maximum NO −2 concentration was only around 2.0 μM at the high CO2 condition (aerated with 5% CO2 in air), furthermore, after 30 h it decreased to no more than 1.0 μM. NO −2 was almost assimilated in 80 h when C. muelleri was cultured at the high CO2 condition with 100 μM NaNO2 as sole nitrogen source. At the high CO2 condition, after 3 h the activity of nitrite reductase was as much as 50% higher than that at the low CO2 condition. It was indicated that enriched CO2 concentration could inhibit nitrite excretion and enhance nitrite assimilation by cells. Therefore, aeration with enriched CO2 might be an effective way to control nitrite content in aquaculture systems.

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Acknowledgements

This study was supported by the Key Laboratory of Bioprocess of Beijing, P.R. China (SYS100100421).

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Authors and Affiliations

  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, P.R. China

    Hanhua Hu

  2. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, P.R. China

    Xu Zhang

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  1. Hanhua Hu
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  2. Xu Zhang
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Correspondence to Hanhua Hu.

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Hu, H., Zhang, X. Nitrite utilization by Chaetoceros muelleri under elevated CO2 concentration. World J Microbiol Biotechnol 24, 891–894 (2008). https://doi.org/10.1007/s11274-007-9553-x

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  • DOI: https://doi.org/10.1007/s11274-007-9553-x

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