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Isolation and characterization of sulphate-reducing bacteria Desulfovibrio vulgaris from Vajreshwari thermal springs in Maharashtra, India

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Abstract

Sulphate-reducing bacteria (SRB) in the thermal springs of Vajreshwari were investigated with combined microbiological and molecular approaches. A sulphate-reducing bacteria medium containing lactate was used for enrichment and isolation, which yielded Gram negative, rod shaped, anaerobic, non-spore forming and motile bacteria capable of reducing sulphate to sulphide. These grew at temperatures ranging from 25 to 55 °C and could use pyruvate, lactate and ethanol as electron donors. Desulfoviridin was detected in all the isolates. The partial 16S rRNA and dissimilatory sulphite reductase (DSR) gene sequences of five representative isolates revealed that the strains belonged to the sulphur reducing bacterial species Desulfovibrio vulgaris.

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Acknowledgements

The authors are grateful to Dr. S. C. Mukherjee, Director, Central Institute of Fisheries Education, for providing all the facilities for carrying out this study. J.G. is thankful to the Indian Council of Agricultural Research, New Delhi, for the Junior Research Fellowship awarded to him.

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Authors and Affiliations

  1. Central Institute of Fisheries Education, Fisheries University Road, Versova, Andheri (W), Mumbai, 400 061, India

    Jeffy George & C. S. Purushothaman

  2. National Centre for Cell Science, University of Pune Campus, Ganeshkind, Pune, 411 007, India

    Yogesh S. Shouche

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  1. Jeffy George
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  2. C. S. Purushothaman
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  3. Yogesh S. Shouche
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Correspondence to C. S. Purushothaman.

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George, J., Purushothaman, C.S. & Shouche, Y.S. Isolation and characterization of sulphate-reducing bacteria Desulfovibrio vulgaris from Vajreshwari thermal springs in Maharashtra, India. World J Microbiol Biotechnol 24, 681–685 (2008). https://doi.org/10.1007/s11274-007-9524-2

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  • DOI: https://doi.org/10.1007/s11274-007-9524-2

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