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Oxalotrophic Paracoccus alcaliphilus isolated from Amorphophallus sp. rhizoplane

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Abstract

A new type of oxalate-utilizing Paracoccus sp. was isolated from Amorphophallus rhizoplane. Basic physiological tests and 16S rDNA sequencing was performed to identify the strain. Optimum growth was observed at 37 °C and pH 8.0 in minimal oxalate (0.5% disodium oxalate) with doubling time of 7 h. Its closest phylogenetic neighbours, as deduced by 16S rDNA-based analysis, were Paracoccus alcaliphilus KCT002, P. alcaliphilus JCM 7364 (TK1015) and P. seriniphilus MBT-A4 with 99.85, 98.35 and 97.31% sequence similarity respectively. Oxalate was metabolized via the serine pathway, as evidenced by the presence of oxalyl-coA reductase, l-serine glyoxylate aminotransferase and hydroxypyruvate reductase.

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Acknowledgements

This work was financially supported by University Grants Commission, India. Anbazhagan Kolandaswamy acknowledges University Grants Commission (UGC) for the fellowship. The authors wish to thank Dr G. Kulandaivelu for Atomic Force Micrograph.

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  1. Department of Biochemistry, Center for Potential in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625 021, India

    Kolandaswamy Anbazhagan, Chelliah Edward Raja & Govindan Sadasivam Selvam

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  1. Kolandaswamy Anbazhagan
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  2. Chelliah Edward Raja
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  3. Govindan Sadasivam Selvam
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Correspondence to Govindan Sadasivam Selvam.

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Anbazhagan, K., Edward Raja, C. & Selvam, G.S. Oxalotrophic Paracoccus alcaliphilus isolated from Amorphophallus sp. rhizoplane. World J Microbiol Biotechnol 23, 1529–1535 (2007). https://doi.org/10.1007/s11274-007-9397-4

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  • DOI: https://doi.org/10.1007/s11274-007-9397-4

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