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Bioconversion of l-phenylalanine into 2-phenylethanol by Kluyveromyces marxianus in grape must cultures

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Abstract

A 23 factorial design was employed to find the best conditions of pH, l-phenylalanine concentration and temperature for the production of 2-phenylethanol by Kluyveromyces marxianus CBS 6556. The cultivation was carried out on grape must, which contains a great amount of nitrogen compounds. Central composite design (CCD) was used for the analysis of treatment combinations. Results showed a second-degree polynomial regression model with good agreement of experimental data, with R 2 = 0.92015 (p < 0.05). The maximum production of 2-phenylethanol was found at pH 7.0, temperature of 37 °C, and a concentration of 3.0 g of l-phenylalanine L−1. Further experiments in bioreactors showed that oxygen concentration is also important to 2-phenylethanol production, with best results obtained at oxygen mass transfer rates of 2.0 h−1.

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References

  • Etschmann MMW, Bluemke W, Sell D, Schrader J (2002) Biotechnology production of 2-phenylethanol. Appl Microbiol Biotechnol 59:1–8

    Article  CAS  Google Scholar 

  • Etschmann MMW, Sell D, Schrader J (2003) Screening of yeasts for the production of the aroma compound 2-phenylethanol in a molasses-based medium. Biotechnol Lett 25:531–536

    Article  CAS  Google Scholar 

  • Etschmann MMW, Sell D, Schrader J (2004) Medium optimization fro the production of the aroma compound 2-phenylethanol using a genetic algorithm. J Mol Catal B Enzym 29:187–193

    Article  CAS  Google Scholar 

  • Fabre CE; Blanc PJ, Goma G (1998) Production of 2-phenylethyl alcohol by Kluyveromyces marxianus. Biotechnol Prog 14:270–264

    Article  Google Scholar 

  • Fabre CE, Condoret J-S, Marty A (1999) Extractive fermentation of aroma with supercritical CO2. Biotechnol Bioeng 70(4):392–400

    Article  Google Scholar 

  • Herández-Orte P, Cacho JF, Ferreira V (2002) Relationship between varietal amino acid profile of grapes and wine aromatic composition. Experiments with model solutions and chemometric study. J Agric Food Chem 50:2891–2899

    Article  Google Scholar 

  • Huang CJ, Lee SL, Chou CC (2000) Production and molar yield of 2-phenylethanol by Pichia fermentans L-5 affected by some medium components. J Biosci Bioeng 90:142–147

    Article  CAS  Google Scholar 

  • Lee WC, Yusof S, Hamid NSA, Baharin BS (2006) Optimizing conditions for enzymatic clarification of banana juice using response surface methodology (RSM). J Food Eng 73(1):16–23

    Article  Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–427

    Article  CAS  Google Scholar 

  • Peynaud E (1984) Knowing and making wine. J.Wiley, New York

    Google Scholar 

  • Rech R, Cassini CF, Secchi A, Ayub MAZ (1999) Utilization of protein-hydrolyzed cheese whey for production of β-galactosidase by Kluyveromyces marxianus. J Industrial Microbiol Biotechnol 23:91–96

    Article  Google Scholar 

  • Serp D, Von Stockar U, Marison IW (2003) Enhancement of 2-phenylethanol productivity by Saccharomyces cerevisiae in two-phase fed-batch fermentations using solvent immobilization. Biotechnol Bioeng 82:103–110

    Article  CAS  Google Scholar 

  • Sinclair CG, Kristiansen B (1987) Fermentations kinetics and modelling. Open University Press, London

    Google Scholar 

  • Stark D, Munch T, Sonnleitner B, Marison IW, Von Stockar U (2002) Extractive bioconversion of 2-phenylethanol from L-phenylalanine by Saccharomyces cerevisiae. Biotechnol Prog 18:514–523

    Article  CAS  Google Scholar 

  • Stark D, Zala D, Münch T, Somnleitner B, Marison IW, Von Stockar U (2003) Inhibition aspects of the bioconversion of L-phenylalanine to 2-phenylethanol by Saccharomyces cerevisiae. Enzyme Microb Technol 32:212–223

    Article  CAS  Google Scholar 

  • Tanyildizi MS, Dursun OZ Elibol M (2005) Optimization of α-amylase production by Bacillus sp. using response surface methodology. Process Biochem 40:2291–2296

    Article  CAS  Google Scholar 

  • Walker T, Morris J, Therlfall R, Main G (2003) Analysis of wine components in Cynthiana and Syrah Wines. J Agric Food Chem 51:1543–1547

    Article  CAS  Google Scholar 

  • Wittman C, Hans M, Bluemke W (2002) Metabolic physiology of aroma-producing Kluyveromyces marxianus. Yeast 19:1351–1363

    Google Scholar 

Download references

Acknowledgements

We would like to thank CNPq and CAPES for their financial support for the research and the scholarship for the first author.

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Authors and Affiliations

  1. Food Science and Technology Institute, Federal University of Rio Grande do Sul State, Av. Bento Gonçalves, 9500, 15090, Porto Alegre , 91501-970, RS, Brazil

    Juliano Garavaglia, Simone Hickmann Flôres & Marco Antônio Záchia Ayub

  2. Chemistry Institute, Federal University of Rio Grande do Sul State, Av. Bento Gonçalves, 9500, 15090, Porto Alegre, 91501-970, RS, Brazil

    Tania Mara Pizzolato & Maria do Carmo Peralba

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  1. Juliano Garavaglia
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  2. Simone Hickmann Flôres
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  3. Tania Mara Pizzolato
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  4. Maria do Carmo Peralba
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  5. Marco Antônio Záchia Ayub
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Correspondence to Marco Antônio Záchia Ayub.

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Garavaglia, J., Flôres, S.H., Pizzolato, T.M. et al. Bioconversion of l-phenylalanine into 2-phenylethanol by Kluyveromyces marxianus in grape must cultures. World J Microbiol Biotechnol 23, 1273–1279 (2007). https://doi.org/10.1007/s11274-007-9361-3

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  • DOI: https://doi.org/10.1007/s11274-007-9361-3

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