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Ethanol production by Saccharomyces cerevisiae and Kluyveromyces marxianus in the presence of orange-peel oil

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Summary

Two ethanologenic yeasts, Saccharomyces cerevisiae and Kluyveromyces marxianus, were used to ferment sugar solutions modeling hydrolyzed Valencia orange peel waste at 37°C. Orange stripper oil produced from orange peel was added in various amounts to determine its effect on ethanol production. The minimum peel oil concentration that inhibited ethanol production was determined after 24, 48 and 72 h and the two yeasts were compared to one another in terms of ethanol yield. Minimum inhibitory peel oil concentrations for ethanol production were 0.05% at 24 h, 0.10% at 48 h, and 0.15% at 72 h for both yeasts. S. cerevisiae produced more ethanol than K. marxianus at each time point.

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Acknowledgments

This manuscript was approved for publication and funding for this study was provided by the Oklahoma Agricultural Experiment Station. The authors would like to acknowledge Miss Sheetal Desai for her assistance in preparing fermentations and collecting data, Mr. Robert Ingraham for his assistance in setting up the GC procedure, and Dr. Bill Widmer for his gift of orange peel oil.

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Authors and Affiliations

  1. Department of Biosystems and Agricultural Engineering, Oklahoma State University, 111 Agricultural Hall, Stillwater, OK, 74078, USA

    Mark R. Wilkins & Lilis Suryawati

  2. Department of Horticulture and Landscape Architecture, Oklahoma State University, 360 Agricultural Hall, Stillwater, OK, 74078, USA

    Niels O. Maness & Donna Chrz

Authors
  1. Mark R. Wilkins
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  2. Lilis Suryawati
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  3. Niels O. Maness
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  4. Donna Chrz
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Correspondence to Mark R. Wilkins.

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Wilkins, M.R., Suryawati, L., Maness, N.O. et al. Ethanol production by Saccharomyces cerevisiae and Kluyveromyces marxianus in the presence of orange-peel oil. World J Microbiol Biotechnol 23, 1161–1168 (2007). https://doi.org/10.1007/s11274-007-9346-2

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  • DOI: https://doi.org/10.1007/s11274-007-9346-2

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