Abstract
Five phosphate-solubilizing bacteria (PSB) used in this study were isolated based on their ability to solubilize tricalcium phosphate (TCP) in Pikovskaya’s medium. Among the tested bacterial strains Burkholderia sp. strain CBPB-HIM showed the highest solubilization (363 μg of soluble P ml−1) activity at 48 h of incubation. Further, this strain has been selected to assess its shelf life in nutrient-amended and -unamended clay, rice bran and rock phosphate (RP) pellet-based granular formulation. The results showed that the maximum viability of bacterium was observed in clay and rice bran (1:1) + 10% RP pellets than clay-RP pellets, irrespective of tested storage temperatures. Further, clay and rice bran (1:1) + 10% RP pellets amended with 1% glucose supported the higher number of cells compared to glycerol-amended and nutrient-unamended pellets. In this carrier solubilization of Morocco rock phosphate (MRP) by Burkholderia sp. strain CBPB-HIM was also investigated. The maximum of water and bicarbonate extractable P (206 and 245 μg P g−1 of pellet respectively) was recorded in clay and rice bran (1:1) + 10% RP pellets amended with 1% glucose and glycerol respectively on day 5 of incubation. Therefore, this study proved the possibility of developing granular inoculant technology combining clay, rice bran and RP as substrates with phosphate-solubilizing Burkholderia.
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Acknowledgements
We thank Sook Jin Kim for assistance in conducting ICP analysis. R. Anandham thanks Korea Research Foundation Grant (KRF-2005-F00009) for awarding a Ph.D fellowship. M. Madhaiyan is supported by the Korea Research Foundation under the foreign scientist and engineers programme. This study was partially supported by the Ministry of Agriculture and Forestry through the Agricultural Research Promotion Centre. We thank V.S. Saravanan for critical reading of this manuscript.
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Anandham, R., Choi, K.H., Indira Gandhi, P. et al. Evaluation of shelf life and rock phosphate solubilization of Burkholderia sp. in nutrient-amended clay, rice bran and rock phosphate-based granular formulation. World J Microbiol Biotechnol 23, 1121–1129 (2007). https://doi.org/10.1007/s11274-006-9342-y
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DOI: https://doi.org/10.1007/s11274-006-9342-y