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Sugar fermentation by Fusarium oxysporum to produce ethanol

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Abstract

As a first step in the research on ethanol production from lignocellulose residues, sugar fermentation by Fusarium oxysporum in oxygen-limited conditions is studied in this work. As a substrate, solutions of arabinose, glucose, xylose and glucose/xylose mixtures are employed. The main kinetic and yield parameters of the process are determined according to a time-dependent model. The microorganism growth is characterized by the maximum specific growth rate and biomass productivity, the substrate consumption is studied through the specific consumption rate and biomass yield, and the product formation via the specific production rate and product yields. In conclusion, F. oxysporum can convert glucose and xylose into ethanol with product yields of 0.38 and 0.25, respectively; when using a glucose/xylose mixture as carbon source, the sugars are utilized sequentially and a maximum value of 0.28 g/g ethanol yield is determined from a 50% glucose/50% xylose mixture. Although fermentation performance by F.␣oxysporum is somewhat lower than that of other fermenting microorganisms, its ability for simultaneous lignocellulose-residue saccharification and fermentation is considered as a potential advantage.

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Abbreviations

b :

biomass productivity (g biomass/l h)

E :

ethanol concentration (g/l)

E T :

theoretical ethanol production (g/l)

q E :

specific ethanol production rate (g ethanol/g biomass h)

\(q^{{\text{D}}}_{{\text{E}}} \) :

specific ethanol production rate by differential method (g ethanol/g biomass h)

q s :

specific substrate consumption rate (g substrate/g biomass h)

\( q^{{\text{D}}}_{{\text{s}}} \) :

specific substrate consumption rate by differential method (g substrate/g biomass h)

s :

substrate concentration (g/l)

s 0 :

initial substrate concentration (g/l)

t :

time (h)

x :

biomass concentration (g/l)

x 0 :

initial biomass concentration (g/l)

Xy :

xylitol concentration (g/l)

Y E/s :

instantaneous ethanol yield (g ethanol/g substrate)

\( Y^{{\text{G}}}_{{E/s}} \) :

overall ethanol yield (g ethanol/g substrate)

Y E/x :

instantaneous ethanol productivity (g ethanol/g biomass)

Y x/s :

instantaneous biomass yield (g biomass/g substrate)

\( Y^{{\text{G}}}_{{x/s}} \) :

overall biomass yield (g biomass/g substrate)

Y Xy/s :

instantaneous xylitol yield (g xylitol/g substrate)

\( Y^{{\text{G}}}_{{{\text{Xy}}/s}} \) :

overall xylitol yield (g xylitol/g substrate)

μ :

specific growth rate (h−1)

μ m :

maximum specific growth rate (h−1)

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Authors and Affiliations

  1. Department of Chemical, Environmental and Materials Engineering, University of Jaén, Campus Las Lagunillas, 23071, Jaén, Spain

    E. Ruiz, I. Romero, M. Moya, S. Sánchez & E. Castro

  2. Department of Chemical Engineering, University of Granada, Campus Fuentenueva, 18071, Granada, Spain

    V. Bravo

Authors
  1. E. Ruiz
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  2. I. Romero
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  3. M. Moya
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  4. S. Sánchez
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  5. V. Bravo
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  6. E. Castro
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Correspondence to E. Castro.

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Ruiz, E., Romero, I., Moya, M. et al. Sugar fermentation by Fusarium oxysporum to produce ethanol. World J Microbiol Biotechnol 23, 259–267 (2007). https://doi.org/10.1007/s11274-006-9222-5

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  • DOI: https://doi.org/10.1007/s11274-006-9222-5

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