Abstract
Tolypothrix scytonemoides subjected to desiccation exhibited increased antioxidant enzyme activities when compared to fresh cells. The activities of catalase (EC 1.11.1.6) and superoxide dismutase (EC 1.15.1.1) were enhanced in desiccated cells by 42.8% and 8.1%, respectively. The isoforms of catalase and superoxide dismutase were detected by activity staining of crude samples separated on native gels. The isoforms of superoxide dismutase were identified based on their sensitivity to hydrogen peroxide and cyanide. The changes in fatty acids and amino acids in fresh and desiccated cells were also investigated and it was found that the quantity of certain fatty acids and amino acids was greater in desiccated cells. Palmitic acid, palmitoleic acid, heptadecanoic acid, linoleic acid, and myristic acid were more in desiccated cells when compared to fresh cells. Desiccated cells synthesized myristoleic acid, eicosenoic acid and behenic acid, acids which were not synthesized by the fresh cells, whereas tricosanoic acid was synthesized by the fresh cells and not by desiccated cells. The levels of lysine, serine, glycine, proline and cysteine were also comparatively greater in the desiccated cells.
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Al-Amoundi OA, Flynn KJ (1989) Effect of nitrate-N incorporation on the composition of the intracellular amino acid pool of N-deprived Tetraselmis marina. British Phycol J 24:53–61
Allakhverdiev SI, Kinoshita M, Inaba M, Suzuki I, Murata N. (2001) Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt induced damage in Synechococcus. Plant Physiol 125:1842–1853
Allakhverdiev SI, Nishiyama Y, Suzuki I, Tasaka Y, Murata N (1999) Genetic engineering of the unsaturation of fatty acids in membrane lipids alters the tolerance of Synechocystis to salt stress. Proc Nat Acad Sci USA 96:5862–5867
Bailly C, Leymarie J, Lehner A, Rousseau S, Come D, Corbineau F (2004) Catalase activity and expression in developing sunflower seeds as related to drying. J Exp Bot 55:475–483
Beauchamp C, Fridovich I (1971) Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Beers RF, Sizer IW (1952) A spectrophotometer method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Bowler C, Mantagu MV, Inze D (1992) Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Mol Biol 43:83–116
Canini A, Civitareale P, Marini S, Grilli-Caiola M, Rotilio G (1992a) Purification of iron superoxide dismutase from the cyanobacterium Anabaena cylindrica Lemm. and localization of the enzyme in heterocysts by immunogold labelling. Planta 187:438–444
Canini A, Grilli Caiola M, Civitareale P, Galiazzo, F (1992b) Superoxide dismutase in symbiont, free-living and wild Anabaena and Nostoc (Nostocales, Cyanophyta). Phycologia 31:223–238
Chongpraditnum P, Mori S, Chino M (1992) Excess copper induces a cytosolic Cu-Zn superoxide dismutase in soybean root. Plant Cell Physiol 33:239–244
Dodds WK, Gudder DA, Mollenhaver D (1995) The ecology of Nostoc. J Phycol 31:2–18
Dohanue JL, Okpodu CM, Cramer CL, Grabau EA, Alscher RG (1997) Response of antioxidants to paraquat in pea leaves. Plant Physiol 113:249–257
Flynn KJ, Al-Amoudi OA (1988) Effects of N deprivation and darkness on composition of free amino acid pool and on amino acid release from diatom Phaeodactylum tricornutum.Botlin. J Exp Marine Biol Ecol 119:131–143
Flynn KJ, Fielder V (1989) Changes in intracellular and extracellular amino acids during the predation of the chlorophyte, Dunaliella primolecta by the heterotrophic dinoflagellate Oxyrrhis marina and the glutamine/glutamate ratio as an indicator of nutrient status in mixed populations. Marine Ecol Prog Series 53:117–127
Flynn KJ, Dickson DMJ, Al-Amoundi OA (1989) The ratio of glutamine: glutamate in microalgae: a biomarker for N-status suitable for use at natural cell densities. J Planktonic Res 11:165–170
Flynn KJ, Garrido JL, Zapata M, Opik H, Hipkin CR (1992) Changes in fatty acids, amino acids and carbon/nitrogen biomass during nitrogen starvation of ammonium-and nitrate -grown Isochrysis galbana. J Appl Phycol 4:95– 104
Foyer CH, Descourvieres P, Kunert KJ (1994) Protection against oxygen radicals : an important defense mechanism studied in transgenic plants. Plant, Cell Environ 17:507–523
Franca MB, Panek AD, Eleutherio ECA (2005) The role of cytoplasmic catalase in dehydration tolerance of Saccharomyces cerevisiae. Cell Stress Chaperones 10:167–170
Gombos Z, Kanervo E, Tsvetkova N, Sakamotor T, Aro EM, Murata N (1997) Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids. Plant Physiol 115:551–559
Gombos Z, Wada H, Murata N (1992) Unsaturation of fatty acids in membrane lipids enhances tolerance of the cyanobacterium Synechocystis PCC 6803 to low temperature photoinhibition. Proc Nat Acad Sci USA 89:9959–9963
Hassan HM, Fridovich I (1977) Enzymatic defenses against the toxicity of oxygen and streptonigrin in Escherichia coli. J Bacteriol 129:1574–1583
Hufleijt M, Tremolieres A, Pineau B, Lang J, Hatheway J, Packer L (1990) Changes in membrane lipid composition during saline growth of the fresh water cyanobacterium Synechococcus 6311. Plant Physiol 94:1512–1521
Khamutov G, Fry IV, Hufleizt ME, Packer L (1990) Membrane lipid composition, fluidity and surface change in response to growth of the fresh water cyanobacterium Synechococcus 6311 under high salinity. Arch Biochem Biophys 277:263–267
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lambeth D (1999) Survey of enzymatic assays by the HPLC method, caltacholamine metabolism. Meth Biochem Anal 38:207–229
Measures JC (1975) Role of amino acids in osmoregulation of non-halophilic bacteria. Nature London 257:398–400
Misra HP, Fridovich I (1972) The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175
Mittler R, Tel-Or E (1991) Oxidative stress responses and shock proteins in the unicellular cyanobacterium Synechococcus R2 (PCC 7942). Arch Microbiol 155:125–130
Olie JJ, Potts M (1986) Purification and biochemical analysis of the cytoplasmic membrane from the dessication-tolerant cyanobacterium Nostoc commune UTEX 584. Appl Environ Microbiol 52:706–710
Paul R, Ahluwalia AS (1987) Biochemical studies during differentiation in two Anabaena spp. Beitr. Pflanzen 61:327–335
Potts M (1999) Mechanisms of desiccation tolerance in cyanobacteria. Eur J Phycol 34:319–328
Potts M, Olie JJ, Nickels JS, Parsons J, White DC (1987) Variation in phospholipid ester-linked fatty acid and carotenoids of desiccated Nostoc commune (cyanobacteria) from different geographic locations. Appl Environ Microbiol 53:4– 9
Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet-B and Ozone – induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136
Rao PSN, Talpasayi ERS (1982) Changes in soluble amino acids, cellular carbohydrates and lipids in brine alga Anabaena fertilissima under salt stress. Indian J Exp Biol 20:765–767
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stainier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J General Microbiol 111:1–61
Ritter D, Yopp JH (1993) Plasma membrane lipid composition of the halophilic cyanobacterium Aphanothece halophytica. Arch Microbiol 159:435–439
Sato N, Murata N, Mura Y, Ueta N (1979) Effects of growth temperature on lipid and fatty acid composition in the blue-green algae, Anabaena variabilis and Anacystis nidulans. Biochimica et Biophysica Acta 572:19–28
Sato N, Murata N (1980) Temperature-induced responses in lipids in the blue-green alga, Anabaena variabilis. Biochimica et Biophysica Acta 619:353–366
Shirkey B, Kovarcik DP, Wright DJ, Wilmoth G, Prickett TF, Helm PF, Gregory EM, Potts M (2000) Active Fe-containing superoxide dismutase and abundant sod F-mRNA in Nostoc commune (cyanobacteria) after years of desiccation. J Bacteriol 182:189–197
Singh SC, Sinha RP, Hader DP (2002) Role of lipids and fatty acids in stress tolerance in cyanobacteria. Acta Protozoologica 41:297–308
Subramanian G, Uma L, Thajuddin N, Sundararaman M, Sekar S, Prabaharan D, Thirunala Sundari T, Malliga P (1999) Manual of Methods in Cyanobacterial Research. National Facility for Marine Cyanobacteria. Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
Tasaka Y, Gombos Z, Nishiyama Y, Mohanty P, Ohba T, Ohki K, Murata N (1996) Targeted mutagenesis of acyl-lipid desaturases in Synechocystis:evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis. EMBO J 15:6416–6425
Tempest DW, Meers JL, Brown CM (1970) Influence of environment on the content and composition of microbial free amino acid pools. J Gen Microbiol 64:171–185
Thompson PA, Harrison PJ, Whyte JNC (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J Phycol 26:278–288
Tindall DR, Yopp JH, Schmid WE, Miller DM (1977) Protein and amino acid composition of the obligate halophile Aphanothece halophytica (Cyanophyta). J Phycol 13:127–133
Tripathi SN, Srivastava P (2001) Presence of stable active oxygen scavenging enzymes superoxide dismutase, ascorbate peroxidase and catalase in a desiccation- tolerant cyanobacterium Lyngbya arboricola under dry state. Current Sci 81:197–200
Wai SN, Mizunoe Y, Takade A, Yoshida S (2000) A comparison of solid and liquid media for resuscitation of starvation- and low temperature- induced nonculturable cells of Aeromonas hydrophila. Arch Microbiol 173:307–310
Woodbury W, Spencer AK, Stahman MA (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal Biochem 44:301–305
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We thank the former Director, CAS in Botany, University of Madras for facilities, Prof. R. Rengasamy for useful discussions and Mrs. Elissa Mutumanje for careful corrections.
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Rajendran, U.M., Kathirvel, E. & Anand, N. Desiccation-induced Changes in Antioxidant Enzymes, Fatty Acids, and Amino Acids in the Cyanobacterium Tolypothrix scytonemoides . World J Microbiol Biotechnol 23, 251–257 (2007). https://doi.org/10.1007/s11274-006-9221-6
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DOI: https://doi.org/10.1007/s11274-006-9221-6