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Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum

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Abstract

An extracellular β-glucosidase (β-glu x) from Sclerotinia sclerotiorum was used as catalyst for the synthesis of gluco-oligosaccharides (GOSs) and alkyl-glucosides. The purified β-glu x was not regiospecific for β(1→4) linkages in either hydrolysis or transglycosylation catalysed-reactions. It efficiently synthesized GOSs from cellobiose, gentiobiose and methyl β-d-glucoside by transglycosylation. At optimal conditions, 119 mg/ml of GOSs (∼ ∼33%) were formed over 9 h from cellobiose as substrate. Alkyl-glucosides were also efficiently synthesized by transglycosylation of cellobiose in presence of different alcohols in biphasic media. However, their concentrations decreased as the size of the alcohol chain increased.

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Aknowledgments

This work was supported by a grant from the French Institute of Cooperation (CMCU 01F0507). The authors would like to thank Dr. Hajlaoui M. Rabeh for providing the fungus.

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Authors and Affiliations

  1. Unité de Génie Biologique, Institut National de Sciences Appliquées et de Technologie (INSAT), BP 676, 1080, Tunis Cedex, Tunisia

    Issam Smaali & Nejib Marzouki

  2. Laboratoire de Biotechnologie et de Chimie Bio-organique, CNRS FRE 2766, Université de La Rochelle, Avenue Michel Crépeau, 17042, La Rochelle, France

    Thierry Maugard & Marie-Dominique Legoy

  3. Institut National de Recherche Scientifique et Technique (INRST), BP 95, 2050, Hammam Lif, Tunisia

    Ferid Limam

Authors
  1. Issam Smaali
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  2. Thierry Maugard
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  3. Ferid Limam
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  4. Marie-Dominique Legoy
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  5. Nejib Marzouki
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Correspondence to Issam Smaali.

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Smaali, I., Maugard, T., Limam, F. et al. Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum . World J Microbiol Biotechnol 23, 145–149 (2007). https://doi.org/10.1007/s11274-006-9185-6

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  • DOI: https://doi.org/10.1007/s11274-006-9185-6

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