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Application of RAPD technique to study polymorphism among Bacillus thuringiensis isolates from Jordan

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Abstract

The soil-borne bacterium Bacillus thuringiensis (Bt) is an important biological agent used against human and plant pests and diseases. Seven Jordanian Bt isolates, which have been analysed for toxicity against important pests, were also differentiated through serotyping. In this study, they were analysed at the molecular level using random amplified polymorphic DNA markers. Five more international strains were incorporated in the analysis. The DNA markers used showed high polymorphism among the isolates tested. However, the data did not align completely with earlier serotyping for most isolates. Therefore, it is recommended to engage several analyses (e.g. biochemical and molecular) when classifying newly surveyed Bt isolates in the world.

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Acknowledgments

This study was funded by a joint grant from Hamdi Mango Center (No. 04200012) and the Deanship of Academic Research, University of Jordan, Jordan (No. 826). The authors would like to thank Miss A. Abdeen and for technical assistance.

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Authors and Affiliations

  1. Plant Biotechnology Lab, Department of Horticulture and Crop Science, Faculty of Agriculture, University of Jordan, Amman, 11942, Jordan

    Monther T. Sadder

  2. Department of Biological Sciences, Faculty of Science, University of Jordan, Amman, 11942, Jordan

    Hala Khyami-Horani

  3. Department of Plant protection, Faculty of Agriculture, University of Jordan, Amman, 11942, Jordan

    Luma Al-Banna

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  1. Monther T. Sadder
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  2. Hala Khyami-Horani
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  3. Luma Al-Banna
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Correspondence to Monther T. Sadder.

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Sadder, M.T., Khyami-Horani, H. & Al-Banna, L. Application of RAPD technique to study polymorphism among Bacillus thuringiensis isolates from Jordan. World J Microbiol Biotechnol 22, 1307–1312 (2006). https://doi.org/10.1007/s11274-006-9177-6

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  • DOI: https://doi.org/10.1007/s11274-006-9177-6

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