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Genetic analysis of glyphosate tolerance in Halomonas variabilis strain HTG7

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Summary

A highly glyphosate-tolerant bacterium strain HTG7 was isolated from glyphosate-polluted soil in north China, and identified as Halomonas variabilis. It was a Gram-negative motile rod giving convex colony. The strain HTG7 could tolerate up to 900 mM glyphosate in minimal medium. The 16S rDNA sequence was amplified by PCR using universal primers. The region essential for glyphosate tolerance was localized to a 3.5-kb fragment from a cosmid library of HTG7. The DNA fragment consisted of one complete open reading frame (ORF) and one partial ORF. The partial ORF was homologous to prephenate dehydrogenase of Pseudomonas aeruginosa PA01. The complete ORF contained the tyrA and aroA genes. Only the 1.35-kb aroA encoding EPSP synthase conferred glyphosate tolerance, and complemented with E. coli aroA mutant ER2799. E. coli JM109 harboring aroA grew well in Mops supplemented with 80 mM glyphosate.

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Acknowledgements

This work was supported by High-technology Research and Development Program of China, and in part financial support from Program on Special Problems and Specific Project for Gene-transferring of China .We thank Shuzhen Ping, Wei Zhang, Yuquan Xu, Li Zou, Rui Zhao and Zhiwei Pan for generously supporting this work.

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Author notes

  1. Zhu Liu

    Present address: Center for Biotechnology and Bioengineering, University of Pittsburgh, Pittsburgh, PA, 15219, USA

Authors and Affiliations

  1. College of Life Science, Sichuan University, 610064, Chengdu, China

    Zhu Liu & Zhirong Yang

  2. Biotechnology research Institute, Chinese Academy of Agriculture Science, 100081, Beijing, China

    Zhu Liu, Wei Lu, Ming Chen & Min Lin

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  1. Zhu Liu
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  2. Wei Lu
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  3. Ming Chen
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  4. Zhirong Yang
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  5. Min Lin
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Correspondence to Min Lin.

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Liu, Z., Lu, W., Chen, M. et al. Genetic analysis of glyphosate tolerance in Halomonas variabilis strain HTG7 . World J Microbiol Biotechnol 22, 681–686 (2006). https://doi.org/10.1007/s11274-005-9090-4

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  • DOI: https://doi.org/10.1007/s11274-005-9090-4

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