Summary
An actinomycete strain (designated Ap1) isolated from the rhizosphere soil of Argania spinosa L. strongly inhibited the growth of two plant pathogens: Fusarium oxysporum f.sp. albedinis and Verticillium dahliae. The spore morphology suggested that the Ap1 strain belonged to the genus Streptomyces. The antifungal compound produced by Ap1 was purified by HPLC and identified as the polyene macrolide, isochainin, by NMR and mass spectroscopy. Ap1 showed normal biosynthesis of isochainin in comparison with S. cellulosae ATTC 12625, in which precursor-directed biosynthesis by feeding ethyl (Z)-16-phenylhexadec-9-enoate to the culture medium is required. In addition, Streptomyces sp. strain Ap1 produces isochainin with a 6.5-fold higher concentration than Streptomyces cellulosae ATTC 12625.
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Acknowledgements
The authors are grateful to Professors José Salas Fernández and Alfredo Fernández Braña, Medicine Faculty, University of Oviedo, Spain for the HPLC isolation and Professor Hartmut Laatsch, Institute for Organic a Biomolecular Chemistry, University of Göttingen, Germany, for the structure determination.
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Bouizgarne, B., El Hadrami, I. & Ouhdouch, Y. Novel Production of Isochainin by a Strain of Streptomyces sp. Isolated from Rhizosphere Soil of the Indigenous Moroccan Plant Argania Spinosa L. World J Microbiol Biotechnol 22, 423–429 (2006). https://doi.org/10.1007/s11274-005-9051-y
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DOI: https://doi.org/10.1007/s11274-005-9051-y