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Comparison of Techniques for the Extraction of Flavonoids from Cultured Cells of Saussurea medusa Maxim

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Room temperature, heat reflux, Soxhlet, ultrasound-assisted and microwave-assisted extractions were employed to extract flavonoids from dry cell cultures of Saussurea medusa Maxim. 24, 20, 6, 0.5 and 0.1 h exposures were necessary for room temperature, Soxhlet, heat reflux, ultrasound-assisted and microwave-assisted extractions to obtain the maximum yield of flavonoids of 3.0%, 4.1%, 3.9%, 3.5% and 4.1%, respectively. Microwave-assisted extraction showed obvious advantages in short duration and high efficiency to extract flavonoids from S. medusa cultured cells.

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References

  • F.J. Chen Y.G. Yang D.X. Zhao (1999) ArticleTitleAdvances in studies of species, habitats distribution and chemical composition of snow lotuses (Saussurea) in China Chinese Bulletin of Botany 16 561–566

    Google Scholar 

  • M.R. Criado I.R. Pereiro R.C. Torrijos (2004) ArticleTitleDetermination of polychlorinated biphenyls in ash using dimethylsulfoxide microwave-assisted extraction followed by solid-phase microextraction Talanta 63 533–540 Occurrence Handle10.1016/j.talanta.2003.11.039

    Article  Google Scholar 

  • M. Eberhardt C.Y. Lee R.H. Liu (2000) ArticleTitleAntioxidant activity of fresh apples Nature 405 903–904 Occurrence Handle1:CAS:528:DC%2BD3cXks1Wmurc%3D

    CAS  Google Scholar 

  • D.P. Fulzele R.K. Satdive (2005) ArticleTitleComparison of techniques for the extraction of the anti-cancer drug camptothecin from Notjapodytes foetida Journal of Chromatography A 1063 9–13 Occurrence Handle10.1016/j.chroma.2004.11.020 Occurrence Handle1:CAS:528:DC%2BD2MXktFSi

    Article  CAS  Google Scholar 

  • Z. Hromadkov A. Ebringerov (2003) ArticleTitleUltrasonic extraction of plant materials – investigation of hemicellulose release from buckwheat hulls Ultrasonics Sonochemistry 10 127–133

    Google Scholar 

  • Y. Huang D.X. Zhao D.P. Lv F. Yan H.G. Chen Q. Zhao (2001) ArticleTitleStudied on the cell suspension culture of Saussarea medusa in a stirred tank bioreactor Chinese Journal of Biotechnology 17 561–565 Occurrence Handle1:CAS:528:DC%2BD3MXot1Gqs7c%3D

    CAS  Google Scholar 

  • J.Z. Jia N.C. Gong M. Du (1989) ArticleTitleStudies on the chemical constituents of Saussurea medusa Maxium Chemical Journal of Chinese Universities 11 202–204

    Google Scholar 

  • Z. Jia M. Tang J. Wu (1999) ArticleTitleThe determination of flavonoid contents in mulberry and their scavenging: effects on superoxide radicals Food Chemistry 64 555–559

    Google Scholar 

  • H. Méndez F. Alava I. Lavilla C. Bendicho (2002) ArticleTitleUltrasonic extraction combined with fast furnace analysis as an improved methodology for total selenium determination in seafood by electrothermal-atomic absorption spectrometry Analytica Chimica Acta 452 217–222

    Google Scholar 

  • T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiologia Plantarum 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm

    CAS  Google Scholar 

  • X.J. Pan H.Z. Liu G.H. Jia H.Z. Liu (2000) ArticleTitleMicrowave-assisted extraction of glycyrrhizic acid from licorice root Biochemical Engineering Journal 5 173–177 Occurrence Handle10.1016/S1369-703X(00)00057-7 Occurrence Handle1:CAS:528:DC%2BD3cXks1Squ70%3D

    Article  CAS  Google Scholar 

  • X.J. Pan G.G. Niu H.Z. Liu (2001) ArticleTitleMicrowave-assisted extraction of tanshinones from Salvia miltiorrhiza bunge with analysis by high performance liquid chromatography Journal of Chromatography A 922 371–375 Occurrence Handle10.1016/S0021-9673(01)00949-9 Occurrence Handle1:CAS:528:DC%2BD3MXlt1Shurc%3D

    Article  CAS  Google Scholar 

  • Y.Y. Shu S.Y. Tey K.S. Wu (2003) ArticleTitleAnalysis of polycyclic aromatic hydrocarbons in airborne particles using open-vessel focused microwave-assisted extraction Analytica Chimica Acta 495 99–108 Occurrence Handle10.1016/j.aca.2003.08.040 Occurrence Handle1:CAS:528:DC%2BD3sXnvFGhtbY%3D

    Article  CAS  Google Scholar 

  • J.M. Xing D.X. Zhao H.C. Ye G.F. Li Z.H. Li (2000) ArticleTitleCell suspension culture of Saussurea medusa in bioreactor Acta Botanica Sinica 42 98–101 Occurrence Handle1:CAS:528:DC%2BD3cXkvVSlt7s%3D

    CAS  Google Scholar 

  • X.F. Yuan B. Zhao Y.C. Wang (2004) ArticleTitleCell culture of Saussurea medusa in a periodically submerged air-lift bioreactor Biochemical Engineering Journal 21 235–239 Occurrence Handle10.1016/j.bej.2004.07.003 Occurrence Handle1:CAS:528:DC%2BD2cXnslGntbk%3D

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P. R. China

    Min Gao & Chun-Zhao Liu

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100093, P. R. China

    Min Gao & Chun-Zhao Liu

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  1. Min Gao
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  2. Chun-Zhao Liu
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Correspondence to Chun-Zhao Liu.

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Gao, M., Liu, CZ. Comparison of Techniques for the Extraction of Flavonoids from Cultured Cells of Saussurea medusa Maxim . World J Microbiol Biotechnol 21, 1461–1463 (2005). https://doi.org/10.1007/s11274-005-6809-1

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  • DOI: https://doi.org/10.1007/s11274-005-6809-1

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